Methyl group acceptance assay for the determination of global DNA methylation levels.

Kenneth Nephew, Curt Balch, David G. Skalnik

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

DNA methylation levels are affected by numerous environmental influences, including diet and xenobiotic exposure, and neoplasia has been firmly associated with genomic hypomethylation and localized hypermethylation of tumor suppressor genes. To reverse methylation-induced gene repression, DNA hypomethylating agents are currently in clinical trials for various malignancies, with two of these now approved for the therapy of myelodysplastic syndrome, and the efficacy of these drugs can be assessed by the monitoring of global DNA methylation levels. Herein, we outline a simple, well-established method for the evaluation of genomic DNA methylation levels, based on the ability of isolated DNA to "accept" radiolabeled methyl groups from S-[3H-methyl] adenosylmethionine, using the bacterial CpG methyltransferase SssI. As this enzyme methylates all unmethylated CpG dinucleotides in the genome, radiolabeled methyl group acceptance is inversely proportional to the level of preexisting methylation. This assay is applicable to a number of translational and basic research questions.

Original languageEnglish
Pages (from-to)35-41
Number of pages7
JournalMethods in molecular biology (Clifton, N.J.)
Volume507
StatePublished - 2009

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DNA Methylation
Methylation
Translational Medical Research
DNA
Myelodysplastic Syndromes
Methyltransferases
Xenobiotics
Tumor Suppressor Genes
Neoplasms
Clinical Trials
Genome
Diet
Enzymes
Pharmaceutical Preparations
Genes
Therapeutics

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Methyl group acceptance assay for the determination of global DNA methylation levels. / Nephew, Kenneth; Balch, Curt; Skalnik, David G.

In: Methods in molecular biology (Clifton, N.J.), Vol. 507, 2009, p. 35-41.

Research output: Contribution to journalArticle

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