Micro-aperture chip system for high-throughput immunomagnetic cell detection

Chun Li Chang, Cagri A. Savran, Shadia Jalal, Daniela Matei

Research output: Chapter in Book/Report/Conference proceedingConference contribution

3 Citations (Scopus)

Abstract

We report a microchip system based on a combination of immunomagnetic separation and microfluidics for high-throughput detection of whole cells. In this system target cells bound to magnetic beads flow parallel to a microchip with flow rates of mLs/min. A magnetic field draws the bead-bound cells toward the microchip which contains apertures that allow passage of unbound beads while trapping the target cells. The cells can be analyzed clearly under a microscope and released from the chip for further analysis by removing the magnetic field. The system was characterized by detecting breast cancer cells (MCF-7) and lung cancer cells (A549) in culture media using anti-EpCAM conjugated magnetic beads. We were able to detect MCF-7 cells with > 90% yield using a flow rate of about 4 mL/min, and A549 cells with 78% yield with 1 mL/min. We also demonstrated high-throughput detection of cells with concentrations as low as 0.8 cells/mL in large sample volumes (12 mL). We expect this system to be useful in a wide variety of settings including detection of rare cells for clinical applications.

Original languageEnglish
Title of host publicationProceedings of IEEE Sensors
DOIs
StatePublished - 2012
Event11th IEEE SENSORS 2012 Conference - Taipei, Taiwan, Province of China
Duration: Oct 28 2012Oct 31 2012

Other

Other11th IEEE SENSORS 2012 Conference
CountryTaiwan, Province of China
CityTaipei
Period10/28/1210/31/12

Fingerprint

Cells
Flow rate
Throughput
Magnetic fields
Parallel flow
Cell culture
Microfluidics
Microscopes

ASJC Scopus subject areas

  • Electrical and Electronic Engineering

Cite this

Micro-aperture chip system for high-throughput immunomagnetic cell detection. / Chang, Chun Li; Savran, Cagri A.; Jalal, Shadia; Matei, Daniela.

Proceedings of IEEE Sensors. 2012. 6411336.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Chang, CL, Savran, CA, Jalal, S & Matei, D 2012, Micro-aperture chip system for high-throughput immunomagnetic cell detection. in Proceedings of IEEE Sensors., 6411336, 11th IEEE SENSORS 2012 Conference, Taipei, Taiwan, Province of China, 10/28/12. https://doi.org/10.1109/ICSENS.2012.6411336
Chang, Chun Li ; Savran, Cagri A. ; Jalal, Shadia ; Matei, Daniela. / Micro-aperture chip system for high-throughput immunomagnetic cell detection. Proceedings of IEEE Sensors. 2012.
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