Micro-aperture chip system for high-throughput immunomagnetic cell detection

Chun Li Chang, Cagri A. Savran, Shadia Jalal, Daniela E. Matei

Research output: Chapter in Book/Report/Conference proceedingConference contribution

3 Scopus citations

Abstract

We report a microchip system based on a combination of immunomagnetic separation and microfluidics for high-throughput detection of whole cells. In this system target cells bound to magnetic beads flow parallel to a microchip with flow rates of mLs/min. A magnetic field draws the bead-bound cells toward the microchip which contains apertures that allow passage of unbound beads while trapping the target cells. The cells can be analyzed clearly under a microscope and released from the chip for further analysis by removing the magnetic field. The system was characterized by detecting breast cancer cells (MCF-7) and lung cancer cells (A549) in culture media using anti-EpCAM conjugated magnetic beads. We were able to detect MCF-7 cells with > 90% yield using a flow rate of about 4 mL/min, and A549 cells with 78% yield with 1 mL/min. We also demonstrated high-throughput detection of cells with concentrations as low as 0.8 cells/mL in large sample volumes (12 mL). We expect this system to be useful in a wide variety of settings including detection of rare cells for clinical applications.

Original languageEnglish (US)
Title of host publicationIEEE SENSORS 2012 - Proceedings
DOIs
StatePublished - Dec 1 2012
Event11th IEEE SENSORS 2012 Conference - Taipei, Taiwan, Province of China
Duration: Oct 28 2012Oct 31 2012

Publication series

NameProceedings of IEEE Sensors

Other

Other11th IEEE SENSORS 2012 Conference
CountryTaiwan, Province of China
CityTaipei
Period10/28/1210/31/12

ASJC Scopus subject areas

  • Electrical and Electronic Engineering

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    Chang, C. L., Savran, C. A., Jalal, S., & Matei, D. E. (2012). Micro-aperture chip system for high-throughput immunomagnetic cell detection. In IEEE SENSORS 2012 - Proceedings [6411336] (Proceedings of IEEE Sensors). https://doi.org/10.1109/ICSENS.2012.6411336