Micro-Raman spectroscopy study of ALVAC virus infected chicken embryo cells

Anupam K. Misra, Lori E. Kamemoto, Ningjie Hu, Ava C. Dykes, Qigui Yu, Pavel V. Zinin, Shiv K. Sharma

Research output: Chapter in Book/Report/Conference proceedingConference contribution

7 Scopus citations

Abstract

Micro- Raman spectroscopic investigation of ALVAC virus and of normal chicken embryo fibroblast cells and the cells infected with ALVAC virus labeled with green fluorescence protein (GFP) were performed with a 785 nm laser. Good quality Micro-Raman spectra of the Alvac II virus were obtained. These spectra show that the ALVAC II virus contains buried tyrosine residues and the coat protein of the virus has α-helical structure. A comparison of Raman spectra of normal and virus infected chicken embryo fibroblast cells revealed that the virus infected cells show additional bands at 535, 928, and 1091 cm-1, respectively, corresponding to δ(C-O-C) glycosidic ring, protein α-helix, and DNA (O-P-O) modes. In addition, the tyrosine resonance double (833 and 855 cm-1) shows reversal in the intensity of the higher-frequency band as compared to the normal cells that can be used to identify the infected cells. In the C-H stretching region, the infected cells show bands with higher intensity as compared to that of the corresponding bands in the normal cells. We also found that the presence of GFP does not affect the Raman spectra of samples when using a 785 nm micro-Raman system because the green fluorescence wavelength of GFP is well below the Stokes-Raman shifted spectral region.

Original languageEnglish (US)
Title of host publicationSmart Biomedical and Physiological Sensor Technology VIII
DOIs
StatePublished - Sep 26 2011
EventSmart Biomedical and Physiological Sensor Technology VIII - Orlando, FL, United States
Duration: Apr 28 2011Apr 28 2011

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume8025
ISSN (Print)1605-7422

Other

OtherSmart Biomedical and Physiological Sensor Technology VIII
CountryUnited States
CityOrlando, FL
Period4/28/114/28/11

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Keywords

  • ALVAC
  • chicken embryo fibroblast cells
  • GFP labeled virus
  • Micro-Raman
  • Virus
  • virus infected cells

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

Cite this

Misra, A. K., Kamemoto, L. E., Hu, N., Dykes, A. C., Yu, Q., Zinin, P. V., & Sharma, S. K. (2011). Micro-Raman spectroscopy study of ALVAC virus infected chicken embryo cells. In Smart Biomedical and Physiological Sensor Technology VIII [80250C] (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 8025). https://doi.org/10.1117/12.887165