Micro-Raman spectroscopy study of ALVAC virus infected chicken embryo cells

Anupam K. Misra, Lori E. Kamemoto, Ningjie Hu, Ava C. Dykes, Andy Yu, Pavel V. Zinin, Shiv K. Sharma

Research output: Chapter in Book/Report/Conference proceedingConference contribution

6 Citations (Scopus)

Abstract

Micro- Raman spectroscopic investigation of ALVAC virus and of normal chicken embryo fibroblast cells and the cells infected with ALVAC virus labeled with green fluorescence protein (GFP) were performed with a 785 nm laser. Good quality Micro-Raman spectra of the Alvac II virus were obtained. These spectra show that the ALVAC II virus contains buried tyrosine residues and the coat protein of the virus has α-helical structure. A comparison of Raman spectra of normal and virus infected chicken embryo fibroblast cells revealed that the virus infected cells show additional bands at 535, 928, and 1091 cm-1, respectively, corresponding to δ(C-O-C) glycosidic ring, protein α-helix, and DNA (O-P-O) modes. In addition, the tyrosine resonance double (833 and 855 cm-1) shows reversal in the intensity of the higher-frequency band as compared to the normal cells that can be used to identify the infected cells. In the C-H stretching region, the infected cells show bands with higher intensity as compared to that of the corresponding bands in the normal cells. We also found that the presence of GFP does not affect the Raman spectra of samples when using a 785 nm micro-Raman system because the green fluorescence wavelength of GFP is well below the Stokes-Raman shifted spectral region.

Original languageEnglish
Title of host publicationProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume8025
DOIs
StatePublished - 2011
EventSmart Biomedical and Physiological Sensor Technology VIII - Orlando, FL, United States
Duration: Apr 28 2011Apr 28 2011

Other

OtherSmart Biomedical and Physiological Sensor Technology VIII
CountryUnited States
CityOrlando, FL
Period4/28/114/28/11

Fingerprint

chickens
Raman Spectrum Analysis
embryos
viruses
Viruses
Raman spectroscopy
Chickens
Embryonic Structures
proteins
Proteins
cells
Fluorescence
Raman scattering
fluorescence
tyrosine
fibroblasts
Fibroblasts
Raman spectra
Tyrosine
Cells

Keywords

  • ALVAC
  • chicken embryo fibroblast cells
  • GFP labeled virus
  • Micro-Raman
  • Virus
  • virus infected cells

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

Cite this

Misra, A. K., Kamemoto, L. E., Hu, N., Dykes, A. C., Yu, A., Zinin, P. V., & Sharma, S. K. (2011). Micro-Raman spectroscopy study of ALVAC virus infected chicken embryo cells. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE (Vol. 8025). [80250C] https://doi.org/10.1117/12.887165

Micro-Raman spectroscopy study of ALVAC virus infected chicken embryo cells. / Misra, Anupam K.; Kamemoto, Lori E.; Hu, Ningjie; Dykes, Ava C.; Yu, Andy; Zinin, Pavel V.; Sharma, Shiv K.

Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 8025 2011. 80250C.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Misra, AK, Kamemoto, LE, Hu, N, Dykes, AC, Yu, A, Zinin, PV & Sharma, SK 2011, Micro-Raman spectroscopy study of ALVAC virus infected chicken embryo cells. in Progress in Biomedical Optics and Imaging - Proceedings of SPIE. vol. 8025, 80250C, Smart Biomedical and Physiological Sensor Technology VIII, Orlando, FL, United States, 4/28/11. https://doi.org/10.1117/12.887165
Misra AK, Kamemoto LE, Hu N, Dykes AC, Yu A, Zinin PV et al. Micro-Raman spectroscopy study of ALVAC virus infected chicken embryo cells. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 8025. 2011. 80250C https://doi.org/10.1117/12.887165
Misra, Anupam K. ; Kamemoto, Lori E. ; Hu, Ningjie ; Dykes, Ava C. ; Yu, Andy ; Zinin, Pavel V. ; Sharma, Shiv K. / Micro-Raman spectroscopy study of ALVAC virus infected chicken embryo cells. Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 8025 2011.
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