Microdissection-based analysis of mature ovarian teratoma

Alexander Vortmeyer, Mojgan Devouassoux-Shisheboran, Guang Li, Victoria Mohr, Fattaneh Tavassoli, Zhengping Zhuang

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

The genotypic features of mature ovarian teratomas (MOTs) are controversial. Early studies detected a homozygous genotype in MOTs suggesting that these tumors are composed of germ cells that have undergone meiosis I. Other studies, however, revealed a heterozygous genotype in a substantial proportion of MOTs suggesting an origin either from premeiotic germ cells or from a somatic cell line. In view of the complex morphology of MOTs and to increase the sensitivity of teratoma genotyping, we applied tissue microdissection before genetic analysis of teratomatous tissue. This approach allowed selective analysis of different heterotopic tissue elements as well as the lymphoid tissues within MOTs the origin of which is unknown. After DNA extraction, the tissue samples were polymerase chain reaction amplified using a random panel of highly informative genetic markers for different chromosomes to evaluate heterozygosity versus homozygosity. In all seven cases that were analyzed, heterotopic tissues consistently revealed a homozygous genotype with several markers; in two cases, heterozygosity was detected with a single marker, indicating a meiotic recombination event. Lymphoid aggregates within MOTs were heterozygous and derived from host tissue rather than from teratomatous growth. However, well differentiated thymic tissue was consistently homozygous, suggesting lymphoid differentiation capability of MOTs. We conclude that potential pitfalls in genotyping of teratomas including meiotic recombination and host cell participation can be avoided by a microdissection-based approach in combination with a panel of genetic markers.

Original languageEnglish (US)
Pages (from-to)987-991
Number of pages5
JournalAmerican Journal of Pathology
Volume154
Issue number4
StatePublished - Jan 1 1999
Externally publishedYes

Fingerprint

Microdissection
Teratoma
Choristoma
Genotype
Genetic Markers
Genetic Recombination
Ovarian Teratoma
Germ Cell and Embryonal Neoplasms
Meiosis
Lymphoid Tissue
Germ Cells
Chromosomes
Cell Line
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Vortmeyer, A., Devouassoux-Shisheboran, M., Li, G., Mohr, V., Tavassoli, F., & Zhuang, Z. (1999). Microdissection-based analysis of mature ovarian teratoma. American Journal of Pathology, 154(4), 987-991.

Microdissection-based analysis of mature ovarian teratoma. / Vortmeyer, Alexander; Devouassoux-Shisheboran, Mojgan; Li, Guang; Mohr, Victoria; Tavassoli, Fattaneh; Zhuang, Zhengping.

In: American Journal of Pathology, Vol. 154, No. 4, 01.01.1999, p. 987-991.

Research output: Contribution to journalArticle

Vortmeyer, A, Devouassoux-Shisheboran, M, Li, G, Mohr, V, Tavassoli, F & Zhuang, Z 1999, 'Microdissection-based analysis of mature ovarian teratoma', American Journal of Pathology, vol. 154, no. 4, pp. 987-991.
Vortmeyer A, Devouassoux-Shisheboran M, Li G, Mohr V, Tavassoli F, Zhuang Z. Microdissection-based analysis of mature ovarian teratoma. American Journal of Pathology. 1999 Jan 1;154(4):987-991.
Vortmeyer, Alexander ; Devouassoux-Shisheboran, Mojgan ; Li, Guang ; Mohr, Victoria ; Tavassoli, Fattaneh ; Zhuang, Zhengping. / Microdissection-based analysis of mature ovarian teratoma. In: American Journal of Pathology. 1999 ; Vol. 154, No. 4. pp. 987-991.
@article{56cfc80aa807493b86f71086353560fc,
title = "Microdissection-based analysis of mature ovarian teratoma",
abstract = "The genotypic features of mature ovarian teratomas (MOTs) are controversial. Early studies detected a homozygous genotype in MOTs suggesting that these tumors are composed of germ cells that have undergone meiosis I. Other studies, however, revealed a heterozygous genotype in a substantial proportion of MOTs suggesting an origin either from premeiotic germ cells or from a somatic cell line. In view of the complex morphology of MOTs and to increase the sensitivity of teratoma genotyping, we applied tissue microdissection before genetic analysis of teratomatous tissue. This approach allowed selective analysis of different heterotopic tissue elements as well as the lymphoid tissues within MOTs the origin of which is unknown. After DNA extraction, the tissue samples were polymerase chain reaction amplified using a random panel of highly informative genetic markers for different chromosomes to evaluate heterozygosity versus homozygosity. In all seven cases that were analyzed, heterotopic tissues consistently revealed a homozygous genotype with several markers; in two cases, heterozygosity was detected with a single marker, indicating a meiotic recombination event. Lymphoid aggregates within MOTs were heterozygous and derived from host tissue rather than from teratomatous growth. However, well differentiated thymic tissue was consistently homozygous, suggesting lymphoid differentiation capability of MOTs. We conclude that potential pitfalls in genotyping of teratomas including meiotic recombination and host cell participation can be avoided by a microdissection-based approach in combination with a panel of genetic markers.",
author = "Alexander Vortmeyer and Mojgan Devouassoux-Shisheboran and Guang Li and Victoria Mohr and Fattaneh Tavassoli and Zhengping Zhuang",
year = "1999",
month = "1",
day = "1",
language = "English (US)",
volume = "154",
pages = "987--991",
journal = "American Journal of Pathology",
issn = "0002-9440",
publisher = "Elsevier Inc.",
number = "4",

}

TY - JOUR

T1 - Microdissection-based analysis of mature ovarian teratoma

AU - Vortmeyer, Alexander

AU - Devouassoux-Shisheboran, Mojgan

AU - Li, Guang

AU - Mohr, Victoria

AU - Tavassoli, Fattaneh

AU - Zhuang, Zhengping

PY - 1999/1/1

Y1 - 1999/1/1

N2 - The genotypic features of mature ovarian teratomas (MOTs) are controversial. Early studies detected a homozygous genotype in MOTs suggesting that these tumors are composed of germ cells that have undergone meiosis I. Other studies, however, revealed a heterozygous genotype in a substantial proportion of MOTs suggesting an origin either from premeiotic germ cells or from a somatic cell line. In view of the complex morphology of MOTs and to increase the sensitivity of teratoma genotyping, we applied tissue microdissection before genetic analysis of teratomatous tissue. This approach allowed selective analysis of different heterotopic tissue elements as well as the lymphoid tissues within MOTs the origin of which is unknown. After DNA extraction, the tissue samples were polymerase chain reaction amplified using a random panel of highly informative genetic markers for different chromosomes to evaluate heterozygosity versus homozygosity. In all seven cases that were analyzed, heterotopic tissues consistently revealed a homozygous genotype with several markers; in two cases, heterozygosity was detected with a single marker, indicating a meiotic recombination event. Lymphoid aggregates within MOTs were heterozygous and derived from host tissue rather than from teratomatous growth. However, well differentiated thymic tissue was consistently homozygous, suggesting lymphoid differentiation capability of MOTs. We conclude that potential pitfalls in genotyping of teratomas including meiotic recombination and host cell participation can be avoided by a microdissection-based approach in combination with a panel of genetic markers.

AB - The genotypic features of mature ovarian teratomas (MOTs) are controversial. Early studies detected a homozygous genotype in MOTs suggesting that these tumors are composed of germ cells that have undergone meiosis I. Other studies, however, revealed a heterozygous genotype in a substantial proportion of MOTs suggesting an origin either from premeiotic germ cells or from a somatic cell line. In view of the complex morphology of MOTs and to increase the sensitivity of teratoma genotyping, we applied tissue microdissection before genetic analysis of teratomatous tissue. This approach allowed selective analysis of different heterotopic tissue elements as well as the lymphoid tissues within MOTs the origin of which is unknown. After DNA extraction, the tissue samples were polymerase chain reaction amplified using a random panel of highly informative genetic markers for different chromosomes to evaluate heterozygosity versus homozygosity. In all seven cases that were analyzed, heterotopic tissues consistently revealed a homozygous genotype with several markers; in two cases, heterozygosity was detected with a single marker, indicating a meiotic recombination event. Lymphoid aggregates within MOTs were heterozygous and derived from host tissue rather than from teratomatous growth. However, well differentiated thymic tissue was consistently homozygous, suggesting lymphoid differentiation capability of MOTs. We conclude that potential pitfalls in genotyping of teratomas including meiotic recombination and host cell participation can be avoided by a microdissection-based approach in combination with a panel of genetic markers.

UR - http://www.scopus.com/inward/record.url?scp=0032923802&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032923802&partnerID=8YFLogxK

M3 - Article

C2 - 10233836

AN - SCOPUS:0032923802

VL - 154

SP - 987

EP - 991

JO - American Journal of Pathology

JF - American Journal of Pathology

SN - 0002-9440

IS - 4

ER -