Microinjection of prostaglandin E2 and muscimol into the preoptic area in conscious rats: Comparison of effects on plasma adrenocorticotrophic hormone (ACTH), body temperature, locomotor activity, and cardiovascular function

Dmitry Zaretsky, Joseph L. Hunt, Maria V. Zaretskaia, Joseph A. DiMicco

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

The preoptic area (POA) is thought to play an important role in thermoregulation and fever. Local application of prostaglandin E2 (PGE2) to this region elicits increases in core body temperature, heart rate, and plasma levels of adrenocorticotrophic hormone (ACTH). Similar effects on body temperature and heart rate have also been reported after local application of the GABAA receptor agonist muscimol to the preoptic area. The purpose of this study was to assess and compare the effects of microinjection of PGE2 and muscimol into the preoptic area in the same chronically instrumented conscious rats on plasma levels of ACTH. Injection of either PGE2 (150 pmol/100 nL) or muscimol (20 or 80 pmol/100 nL) into the same sites in the preoptic area evoked increases in body temperature, heart rate, blood pressure, and plasma levels of ACTH, while significant increases in locomotor activity were apparent only after muscimol. These data confirm and extend previous findings and support the notion that neurons in the region of the preoptic area exert tonic inhibition on downstream mechanisms capable of increasing the activity of the hypothalamic-pituitary-adrenal (HPA) axis as well as sympathetic thermogenic and cardiac activity.

Original languageEnglish
Pages (from-to)291-296
Number of pages6
JournalNeuroscience Letters
Volume397
Issue number3
DOIs
StatePublished - Apr 24 2006

Fingerprint

Muscimol
Preoptic Area
Microinjections
Locomotion
Body Temperature
Dinoprostone
Adrenocorticotropic Hormone
Heart Rate
GABA-A Receptor Agonists
Body Temperature Regulation
Fever
Blood Pressure
Neurons
Injections

Keywords

  • Blood pressure
  • Body temperature
  • Fever
  • Heart rate
  • Hypothalamus

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

@article{f76bc490726a4cd4a0efd8800757427d,
title = "Microinjection of prostaglandin E2 and muscimol into the preoptic area in conscious rats: Comparison of effects on plasma adrenocorticotrophic hormone (ACTH), body temperature, locomotor activity, and cardiovascular function",
abstract = "The preoptic area (POA) is thought to play an important role in thermoregulation and fever. Local application of prostaglandin E2 (PGE2) to this region elicits increases in core body temperature, heart rate, and plasma levels of adrenocorticotrophic hormone (ACTH). Similar effects on body temperature and heart rate have also been reported after local application of the GABAA receptor agonist muscimol to the preoptic area. The purpose of this study was to assess and compare the effects of microinjection of PGE2 and muscimol into the preoptic area in the same chronically instrumented conscious rats on plasma levels of ACTH. Injection of either PGE2 (150 pmol/100 nL) or muscimol (20 or 80 pmol/100 nL) into the same sites in the preoptic area evoked increases in body temperature, heart rate, blood pressure, and plasma levels of ACTH, while significant increases in locomotor activity were apparent only after muscimol. These data confirm and extend previous findings and support the notion that neurons in the region of the preoptic area exert tonic inhibition on downstream mechanisms capable of increasing the activity of the hypothalamic-pituitary-adrenal (HPA) axis as well as sympathetic thermogenic and cardiac activity.",
keywords = "Blood pressure, Body temperature, Fever, Heart rate, Hypothalamus",
author = "Dmitry Zaretsky and Hunt, {Joseph L.} and Zaretskaia, {Maria V.} and DiMicco, {Joseph A.}",
year = "2006",
month = "4",
day = "24",
doi = "10.1016/j.neulet.2005.12.032",
language = "English",
volume = "397",
pages = "291--296",
journal = "Neuroscience Letters",
issn = "0304-3940",
publisher = "Elsevier Ireland Ltd",
number = "3",

}

TY - JOUR

T1 - Microinjection of prostaglandin E2 and muscimol into the preoptic area in conscious rats

T2 - Comparison of effects on plasma adrenocorticotrophic hormone (ACTH), body temperature, locomotor activity, and cardiovascular function

AU - Zaretsky, Dmitry

AU - Hunt, Joseph L.

AU - Zaretskaia, Maria V.

AU - DiMicco, Joseph A.

PY - 2006/4/24

Y1 - 2006/4/24

N2 - The preoptic area (POA) is thought to play an important role in thermoregulation and fever. Local application of prostaglandin E2 (PGE2) to this region elicits increases in core body temperature, heart rate, and plasma levels of adrenocorticotrophic hormone (ACTH). Similar effects on body temperature and heart rate have also been reported after local application of the GABAA receptor agonist muscimol to the preoptic area. The purpose of this study was to assess and compare the effects of microinjection of PGE2 and muscimol into the preoptic area in the same chronically instrumented conscious rats on plasma levels of ACTH. Injection of either PGE2 (150 pmol/100 nL) or muscimol (20 or 80 pmol/100 nL) into the same sites in the preoptic area evoked increases in body temperature, heart rate, blood pressure, and plasma levels of ACTH, while significant increases in locomotor activity were apparent only after muscimol. These data confirm and extend previous findings and support the notion that neurons in the region of the preoptic area exert tonic inhibition on downstream mechanisms capable of increasing the activity of the hypothalamic-pituitary-adrenal (HPA) axis as well as sympathetic thermogenic and cardiac activity.

AB - The preoptic area (POA) is thought to play an important role in thermoregulation and fever. Local application of prostaglandin E2 (PGE2) to this region elicits increases in core body temperature, heart rate, and plasma levels of adrenocorticotrophic hormone (ACTH). Similar effects on body temperature and heart rate have also been reported after local application of the GABAA receptor agonist muscimol to the preoptic area. The purpose of this study was to assess and compare the effects of microinjection of PGE2 and muscimol into the preoptic area in the same chronically instrumented conscious rats on plasma levels of ACTH. Injection of either PGE2 (150 pmol/100 nL) or muscimol (20 or 80 pmol/100 nL) into the same sites in the preoptic area evoked increases in body temperature, heart rate, blood pressure, and plasma levels of ACTH, while significant increases in locomotor activity were apparent only after muscimol. These data confirm and extend previous findings and support the notion that neurons in the region of the preoptic area exert tonic inhibition on downstream mechanisms capable of increasing the activity of the hypothalamic-pituitary-adrenal (HPA) axis as well as sympathetic thermogenic and cardiac activity.

KW - Blood pressure

KW - Body temperature

KW - Fever

KW - Heart rate

KW - Hypothalamus

UR - http://www.scopus.com/inward/record.url?scp=33644986992&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33644986992&partnerID=8YFLogxK

U2 - 10.1016/j.neulet.2005.12.032

DO - 10.1016/j.neulet.2005.12.032

M3 - Article

C2 - 16406311

AN - SCOPUS:33644986992

VL - 397

SP - 291

EP - 296

JO - Neuroscience Letters

JF - Neuroscience Letters

SN - 0304-3940

IS - 3

ER -