MicroRNA-31 functions as an oncogenic microRNA in mouse and human lung cancer cells by repressing specific tumor suppressors

Xi Liu, Lorenzo F. Sempere, Haoxu Ouyang, Vincent A. Memoli, Angeline S. Andrew, Yue Luo, Eugene Demidenko, Murray Korc, Wei Shi, Meir Preis, Konstantin H. Dragnev, Hua Li, James Direnzo, Mads Bak, Sarah J. Freemantle, Sakari Kauppinen, Ethan Dmitrovsky

Research output: Contribution to journalArticle

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Abstract

MicroRNAs (miRNAs) regulate gene expression. It has been suggested that obtaining miRNA expression profiles can improve classification, diagnostic, and prognostic information in oncology. Here, we sought to comprehensively identify the miRNAs that are overexpressed in lung cancer by conducting miRNA microarray expression profiling on normal lung versus adjacent lung cancers from transgenic mice. We found that miR-136, miR-376a, and miR-31 were each prominently overexpressed in murine lung cancers. Real-time RT-PCR and in situ hybridization (ISH) assays confirmed these miRNA expression profiles in paired normal-malignant lung tissues from mice and humans. Engineered knockdown of miR-31, but not other highlighted miRNAs, substantially repressed lung cancer cell growth and tumorigenicity in a dose-dependent manner. Using a bioinformatics approach, we identified miR-31 target mRNAs and independently confirmed them as direct targets in human and mouse lung cancer cell lines. These targets included the tumor-suppressive genes large tumor suppressor 2 (LATS2) and PP2A regulatory subunit B alpha isoform (PPP2R2A), and expression of each was augmented by miR-31 knockdown. Their engineered repression antagonized miR-31-mediated growth inhibition. Notably, miR-31 and these target mRNAs were inversely expressed in mouse and human lung cancers, underscoring their biologic relevance. The clinical relevance of miR-31 expression was further independently and comprehensively validated using an array containing normal and malignant human lung tissues. Together, these findings revealed that miR-31 acts as an oncogenic miRNA (oncomir) in lung cancer by targeting specific tumor suppressors for repression.

Original languageEnglish (US)
Pages (from-to)1298-1309
Number of pages12
JournalJournal of Clinical Investigation
Volume120
Issue number4
DOIs
StatePublished - Apr 1 2010
Externally publishedYes

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MicroRNAs
Lung Neoplasms
Neoplasms
Lung
Messenger RNA
Growth
Computational Biology
Tumor Suppressor Genes
Transgenic Mice
In Situ Hybridization
Real-Time Polymerase Chain Reaction
Protein Isoforms
Gene Expression
Cell Line

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Liu, X., Sempere, L. F., Ouyang, H., Memoli, V. A., Andrew, A. S., Luo, Y., ... Dmitrovsky, E. (2010). MicroRNA-31 functions as an oncogenic microRNA in mouse and human lung cancer cells by repressing specific tumor suppressors. Journal of Clinical Investigation, 120(4), 1298-1309. https://doi.org/10.1172/JCI39566

MicroRNA-31 functions as an oncogenic microRNA in mouse and human lung cancer cells by repressing specific tumor suppressors. / Liu, Xi; Sempere, Lorenzo F.; Ouyang, Haoxu; Memoli, Vincent A.; Andrew, Angeline S.; Luo, Yue; Demidenko, Eugene; Korc, Murray; Shi, Wei; Preis, Meir; Dragnev, Konstantin H.; Li, Hua; Direnzo, James; Bak, Mads; Freemantle, Sarah J.; Kauppinen, Sakari; Dmitrovsky, Ethan.

In: Journal of Clinical Investigation, Vol. 120, No. 4, 01.04.2010, p. 1298-1309.

Research output: Contribution to journalArticle

Liu, X, Sempere, LF, Ouyang, H, Memoli, VA, Andrew, AS, Luo, Y, Demidenko, E, Korc, M, Shi, W, Preis, M, Dragnev, KH, Li, H, Direnzo, J, Bak, M, Freemantle, SJ, Kauppinen, S & Dmitrovsky, E 2010, 'MicroRNA-31 functions as an oncogenic microRNA in mouse and human lung cancer cells by repressing specific tumor suppressors', Journal of Clinical Investigation, vol. 120, no. 4, pp. 1298-1309. https://doi.org/10.1172/JCI39566
Liu, Xi ; Sempere, Lorenzo F. ; Ouyang, Haoxu ; Memoli, Vincent A. ; Andrew, Angeline S. ; Luo, Yue ; Demidenko, Eugene ; Korc, Murray ; Shi, Wei ; Preis, Meir ; Dragnev, Konstantin H. ; Li, Hua ; Direnzo, James ; Bak, Mads ; Freemantle, Sarah J. ; Kauppinen, Sakari ; Dmitrovsky, Ethan. / MicroRNA-31 functions as an oncogenic microRNA in mouse and human lung cancer cells by repressing specific tumor suppressors. In: Journal of Clinical Investigation. 2010 ; Vol. 120, No. 4. pp. 1298-1309.
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AU - Demidenko, Eugene

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AU - Direnzo, James

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