MicroRNA 362-3p Reduces hERG-related Current and Inhibits Breast Cancer Cells Proliferation

Abdullah A. Assiri, Noha Mourad, Minghai Shao, Patrick Kiel, Wanqing Liu, Todd C. Skaar, Brian R. Overholser

Research output: Contribution to journalArticle

Abstract

BACKGROUND/AIM: hERG potassium channels enhance tumor invasiveness and breast cancer proliferation. MicroRNA (miRNA) dysregulation during cancer controls gene regulation. The objective of this study was to identify miRNAs that regulate hERG expression in breast cancer. MATERIALS AND METHODS: Putative miRNAs targeting hERG were identified by bioinformatic approaches and screened using a 3'UTR luciferase assay. Functional assessments of endogenous hERG regulation were made using whole-cell electrophysiology, proliferation assays, and cell-cycle analyses following miRNA, hERG siRNA, or control transfection. RESULTS: miR-362-3p targeted hERG 3'UTR and was associated with higher survival rates in patients with breast cancer (HR=0.39, 95%CI=0.18-0.82). Enhanced miR-362-3p expression reduced hERG expression, peak current, and cell proliferation in cultured breast cancer cells (p<0.05). CONCLUSION: miR-362-3p mediates the transcriptional regulation of hERG and is associated with survival in breast cancer. The potential for miR-362-3p to serve as a biomarker and inform therapeutic strategies warrants further investigation.

Original languageEnglish (US)
Pages (from-to)433-442
Number of pages10
JournalCancer genomics & proteomics
Volume16
Issue number6
DOIs
StatePublished - Nov 1 2019

Fingerprint

Cell proliferation
MicroRNAs
Cell Proliferation
Breast Neoplasms
3' Untranslated Regions
Assays
Functional assessment
Cells
Electrophysiology
Potassium Channels
Biomarkers
Bioinformatics
Luciferases
Gene expression
Small Interfering RNA
Neoplasm Genes
Tumors
Computational Biology
Transfection
Cell Cycle

Keywords

  • breast cancer
  • hERG
  • MicroRNA
  • miR-362-3p
  • voltage-gated potassium channels

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cancer Research

Cite this

MicroRNA 362-3p Reduces hERG-related Current and Inhibits Breast Cancer Cells Proliferation. / Assiri, Abdullah A.; Mourad, Noha; Shao, Minghai; Kiel, Patrick; Liu, Wanqing; Skaar, Todd C.; Overholser, Brian R.

In: Cancer genomics & proteomics, Vol. 16, No. 6, 01.11.2019, p. 433-442.

Research output: Contribution to journalArticle

Assiri, Abdullah A. ; Mourad, Noha ; Shao, Minghai ; Kiel, Patrick ; Liu, Wanqing ; Skaar, Todd C. ; Overholser, Brian R. / MicroRNA 362-3p Reduces hERG-related Current and Inhibits Breast Cancer Cells Proliferation. In: Cancer genomics & proteomics. 2019 ; Vol. 16, No. 6. pp. 433-442.
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