Microvascular smooth muscle cell quantitation from scanning electron microscopic preparations

Vincent H. Gattone, Bryan G. Miller, Andrew P. Evan

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

A morphometric method to analyze scanning electron micrographs (SEM) of microvascular smooth muscle cells (SMC) is validated using intestinal arterioles. Features easily obtained from these microvasculature preparations are counted (number of tapers and number of complete wraps in the central 87% of the vessel, which is one‐third of the vessel's circumference) or measured (vessel diameter and vessel segment length). These data allow the determination of wraps around the vessel per SMC, cell length, and cell width, which are not different from either the values as determined by circumferential examination and quantitation of the vascular segment or an alternative SEM quantitation method (Miller et al., 1986). The method described herein provides a relatively easy way to determine microvascular SMC parameters.

Original languageEnglish (US)
Pages (from-to)443-447
Number of pages5
JournalThe Anatomical Record
Volume216
Issue number3
DOIs
StatePublished - Nov 1986

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ASJC Scopus subject areas

  • Anatomy
  • Agricultural and Biological Sciences (miscellaneous)

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