Minimal effect of acute experimental hepatitis induced by lipopolysaccharide/D-galactosamine on biotransformation in rats

Hossam El Din M Omar, Ruth A. Sanders, John B. Watkins

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

When administered with D-galactosamine, lipopolysaccharide endotoxins produce a good experimental animal model of hepatitis. This galactosamine plus endotoxin model has been used widely, but the acute effect of this fixed combination of two chemicals on hepatic and extrahepatic biotransformation has not been determined. Therefore, either 2 or 4 hr after a single intraperitoneal dose of 300 mg/kg galactosamine plus 30 μg/kg lipopolysaccharide was administered, serum, liver, kidney, intestine, and spleen were collected. Serum enzymes (alanine and aspartate aminotransferases, sorbitol dehydrogenase, and γ-glutamyltranspeptidase) were elevated dramatically 2 and 4 hr after treatment. Cytochrome P450 monooxygenase activity toward benzo[a]pyrene was increased in kidney 4 hr after treatment, whereas dealkylation of 7-methoxycoumarin or 7-ethoxyresorufin was unchanged in any tissue at either time point. An increase in UDP-glucuronosyltransferase activity toward 4-methylumbelliferone and 4-hydroxybiphenyl was noted in the intestine. Conjugation of 1-chloro-2,4-dinitrobenzene with glutathione was increased in intestine and spleen 2 hr after treatment. γ-Glutamyltranspeptidase activity was unaltered in all sues studied. Reduced glutathione concentrations were increased significantly by different amounts depending on which organs were studied 2 or 4 hr after treatment. These results indicate that galactosamine/lipopolysaccharide-induced liver injury is not accompanied by major effects on the examined biotransformation reactions.

Original languageEnglish
Pages (from-to)1921-1924
Number of pages4
JournalBiochemical Pharmacology
Volume52
Issue number12
DOIs
StatePublished - Dec 24 1996

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Galactosamine
Biotransformation
Hepatitis
Lipopolysaccharides
Rats
Intestines
Endotoxins
Liver
Glutathione
Animal Hepatitis
Spleen
Hymecromone
L-Iditol 2-Dehydrogenase
Dealkylation
Kidney
Dinitrochlorobenzene
Glucuronosyltransferase
Benzo(a)pyrene
Aspartate Aminotransferases
Mixed Function Oxygenases

Keywords

  • cytochrome P450 monooxygenases
  • endotoxin
  • galactosamine
  • intestine
  • kidney
  • liver
  • spleen
  • UDP-glucuronosyltransferase

ASJC Scopus subject areas

  • Pharmacology

Cite this

Minimal effect of acute experimental hepatitis induced by lipopolysaccharide/D-galactosamine on biotransformation in rats. / Omar, Hossam El Din M; Sanders, Ruth A.; Watkins, John B.

In: Biochemical Pharmacology, Vol. 52, No. 12, 24.12.1996, p. 1921-1924.

Research output: Contribution to journalArticle

Omar, Hossam El Din M ; Sanders, Ruth A. ; Watkins, John B. / Minimal effect of acute experimental hepatitis induced by lipopolysaccharide/D-galactosamine on biotransformation in rats. In: Biochemical Pharmacology. 1996 ; Vol. 52, No. 12. pp. 1921-1924.
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