MiR-9 controls chemotactic activity of cord blood CD34+ cells by repressing CXCR4 expression

Tae Won Ha, Hyun Soo Kang, Tae Hee Kim, Ji Hyun Kwon, Hyun Kyu Kim, Aeli Ryu, Hyeji Jeon, Jaeseok Han, Hal E. Broxmeyer, Yongsung Hwang, Yun Kyung Lee, Man Ryul Lee

Research output: Contribution to journalArticle

Abstract

Improved approaches for promoting umbilical cord blood (CB) hematopoietic stem cell (HSC) homing are clinically important to enhance engraftment of CB-HSCs. Clinical transplantation of CB-HSCs is used to treat a wide range of disorders. However, an improved understanding of HSC chemotaxis is needed for facilitation of the engraftment process. We found that ectopic overexpression of miR-9 and antisense-miR-9 respectively down- and up-regulated C-X-C chemokine receptor type 4 (CXCR4) expression in CB-CD34+ cells as well as in 293T and TF-1 cell lines. Since CXCR4 is a specific receptor for the stromal cell derived factor-1 (SDF-1) chemotactic factor, we investigated whether sense miR-9 and antisense miR-9 influenced CXCR4-mediated chemotactic mobility of primary CB CD34+ cells and TF-1 cells. Ectopic overexpression of sense miR-9 and antisense miR-9 respectively down- and up-regulated SDF-1-mediated chemotactic cell mobility. To our knowledge, this study is the first to report that miR-9 may play a role in regulating CXCR4 expression and SDF-1-mediated chemotactic activity of CB CD34+ cells.

Original languageEnglish (US)
Pages (from-to)187-195
Number of pages9
JournalInternational Journal of Stem Cells
Volume11
Issue number2
DOIs
StatePublished - Nov 1 2018

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Keywords

  • CXCR4
  • Cell migration
  • Cord blood
  • MicroRNA

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology

Cite this

Ha, T. W., Kang, H. S., Kim, T. H., Kwon, J. H., Kim, H. K., Ryu, A., Jeon, H., Han, J., Broxmeyer, H. E., Hwang, Y., Lee, Y. K., & Lee, M. R. (2018). MiR-9 controls chemotactic activity of cord blood CD34+ cells by repressing CXCR4 expression. International Journal of Stem Cells, 11(2), 187-195. https://doi.org/10.15283/ijsc18057