Mispolarization of desmosomal proteins and altered intercellular adhesion in autosomal dominant polycystic kidney disease

Melina Silberberg, Audra J. Charron, Robert Bacallao, Angela Wandinger-Ness

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Polycystin-1, the product of the major gene mutated in autosomal dominant polycystic kidney disease (ADPKD), has been shown to associate with multiple epithelial cell junctions. Our hypothesis is that polycystin-1 is an important protein for the initial establishment of cell-cell junctions and maturation of the cell and that polycystin-1 localization is dependent on the degree of cell polarization. Using laser-scanning confocal microscopy and two models of cell polarization, polycystin-1 and desmosomes were found to colocalize during the initial establishment of cell-cell contact when junctions were forming. However, colocalization was lost in confluent monolayers. Parallel morphological and biochemical evaluations revealed a profound mispolarization of desmosomal components to both the apical and basolateral domains in primary ADPKD cells and tissue. Studies of the intermediate filament network associated with desmosomes showed that there is a decrease in cytokeratin levels and an abnormal expression of the mesenchymal protein vimentin in the disease. Moreover, we show for the first time that the structural alterations seen in adherens and desmosomal junctions have a functional impact, leaving the ADPKD cells with weakened cell-cell adhesion. In conclusion, in this paper we show that polycystin-1 transiently colocalizes with desmosomes and that desmosomal proteins are mislocalized as a consequence of polycystin-1 mutation.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Renal Physiology
Volume288
Issue number6 57-6
DOIs
StatePublished - Jun 2005

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Autosomal Dominant Polycystic Kidney
Desmosomes
Proteins
Intercellular Junctions
Adherens Junctions
Intermediate Filaments
Vimentin
Keratins
Cell Adhesion
Confocal Microscopy
polycystic kidney disease 1 protein
Epithelial Cells
Mutation

Keywords

  • Calcium switch
  • Cytoskeleton
  • Dispase assay
  • Polarity
  • Polycystin

ASJC Scopus subject areas

  • Physiology

Cite this

Mispolarization of desmosomal proteins and altered intercellular adhesion in autosomal dominant polycystic kidney disease. / Silberberg, Melina; Charron, Audra J.; Bacallao, Robert; Wandinger-Ness, Angela.

In: American Journal of Physiology - Renal Physiology, Vol. 288, No. 6 57-6, 06.2005.

Research output: Contribution to journalArticle

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