Mitochondria-mediated and p53-associated apoptosis induced in human cancer cells by a novel selenophene derivative, D-501036

Her Shyong Shiah, Wan Shu Lee, Shin Hun Juang, Pao Chiung Hong, Chia Chi Lung, Ching Jer Chang, Kai Ming Chou, Jang Yang Chang

Research output: Contribution to journalArticle

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Abstract

D-501036 [2,5-bis(5-hydroxymethyl-2-selenienyl)-3-hydroxymethyl-N-methylpyrrol], a novel selenophene derivative, is a highly potent cytotoxic agent with broad spectrum antitumor activity. The present study was undertaken to explore the mechanism(s) through which D-501036 exerts its action mode on the cancer cell death. D-501036 was found to suppress the growth of KB and HepG2 cells in an irreversible manner. The results of annexin-V assays and PARP cleavage studies were consistent with the D-501036-induced apoptosis. Findings provided a strong support for the induction of mitochondria-mediated apoptosis by this drug. The examination of two canonical pathways of initiation caspases, those for caspases -8 and -9, revealed that caspase-9 protein and the activities of caspases -9 and -3 were increased in a dose- and time-dependent manner. The concentrations of Fas/Fas-L and procaspase-8 and the activity of caspase-8 were not altered. Furthermore, the mitochondrial membrane potential permeability and the release of cytochrome c to the cytosol were both increased by D-501036. The concentrations of the pro-apoptotic protein Bax and translocation of Bax from the cytosol to the mitochondria were increased in response to D-501036, whereas the concentrations of the anti-apoptotic protein Bcl-2 were decreased. Two DNA damage-related pro-apoptotic proteins, Puma and Noxa, were upregulated in a dose- and time-dependent manner. These pro-apoptotic and anti-apoptotic proteins are downstream effectors of p53. Accordingly, the phosphorylated and total forms of p53 were induced and p53 was translocated from the cytosol to the mitochondria in response to D-501036 treatment. Collectively, we conclude that D-501036 induces cellular apoptosis through the p53-associated mitochondrial pathway.

Original languageEnglish (US)
Pages (from-to)610-619
Number of pages10
JournalBiochemical Pharmacology
Volume73
Issue number5
DOIs
StatePublished - Mar 1 2007
Externally publishedYes

Fingerprint

Mitochondria
Cells
Apoptosis
Derivatives
Apoptosis Regulatory Proteins
Caspase 9
Caspase 8
Neoplasms
Cytosol
Puma
Noxae
KB Cells
2,5-bis(5-hydroxymethyl-2-selenienyl)-3-hydroxymethyl-N-methylpyrrole
Mitochondrial Membrane Potential
Annexin A5
Cytotoxins
Hep G2 Cells
Protein Transport
Cell death
Caspases

Keywords

  • Apoptosis
  • Caspase
  • Mitochondria
  • p53
  • Selenophene derivative

ASJC Scopus subject areas

  • Pharmacology

Cite this

Mitochondria-mediated and p53-associated apoptosis induced in human cancer cells by a novel selenophene derivative, D-501036. / Shiah, Her Shyong; Lee, Wan Shu; Juang, Shin Hun; Hong, Pao Chiung; Lung, Chia Chi; Chang, Ching Jer; Chou, Kai Ming; Chang, Jang Yang.

In: Biochemical Pharmacology, Vol. 73, No. 5, 01.03.2007, p. 610-619.

Research output: Contribution to journalArticle

Shiah, Her Shyong ; Lee, Wan Shu ; Juang, Shin Hun ; Hong, Pao Chiung ; Lung, Chia Chi ; Chang, Ching Jer ; Chou, Kai Ming ; Chang, Jang Yang. / Mitochondria-mediated and p53-associated apoptosis induced in human cancer cells by a novel selenophene derivative, D-501036. In: Biochemical Pharmacology. 2007 ; Vol. 73, No. 5. pp. 610-619.
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AU - Hong, Pao Chiung

AU - Lung, Chia Chi

AU - Chang, Ching Jer

AU - Chou, Kai Ming

AU - Chang, Jang Yang

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