Emetine (at 100 μg/ml) blocks the incorporation of 3H-leucine into the total protein of slices from rat cerebral cortex by > 95%; the block on the synthesis of cytosol proteins is quantitative. Under these conditions protein synthesis resistant to emetine is localized in a mitochondrial fraction that is sensitive to chloramphenicol at 100 μg/ml to the extent of ∼ 70%. We infer that some 15% of the proteins of cerebral cortex mitochondria formed during a 60 min period have been provided by their own protein synthesizing system.
|Original language||English (US)|
|Number of pages||6|
|Journal||Biochemical and Biophysical Research Communications|
|State||Published - Feb 5 1971|
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology