Mitochondrial respiratory mutants in yeast inhibit glycogen accumulation by blocking activation of glycogen synthase

Ruojing Yang, Kristin Chun, Ronald Wek

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Control of glycogen synthase activity by protein phosphorylation is important for regulating the synthesis of glycogen. In this report, we describe a regulatory linkage between the ability of yeast cells to respire and activation of glycogen synthase. Strains containing respiration-deficient mutations in genes such as COQ3, required for the synthesis of coenzyme Q, were reduced in their ability to accumulate glycogen in response to limiting glucose. This lowered glycogen accumulation results from inactivation of the rate-determining enzyme, glycogen synthase (Gsy2p). Reduced glycogen synthase activity is coincident with lowered glucose 6-phosphate and ATP levels in the respiration-deficient cells deprived of glucose. Alanine substitutions of three previously characterized phosphorylation sites in Gsy2p, Ser650, Ser- 654, or Thr-667, each suppressed the glycogen defect in cells unable to respire, suggesting that inactivation of this enzyme is mediated by phosphorylation of these residues. Inactivation of glycogen synthase requires the RAS signaling pathway that controls cAMP-dependent protein kinase and is independent of Pho85p previously identified as a Gsy2p kinase. These results suggest that yeast cells unable to shift from a fermentative to a respiratory metabolic regimen block accumulation of glycogen by inactivating Gsy2p through protein phosphorylation.

Original languageEnglish
Pages (from-to)31337-31344
Number of pages8
JournalJournal of Biological Chemistry
Volume273
Issue number47
DOIs
StatePublished - Nov 20 1998

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Glycogen Synthase
Glycogen
Phosphorylation
Yeast
Yeasts
Chemical activation
Cells
Cell Respiration
Glucose
Glucose-6-Phosphate
Ubiquinone
Enzymes
Cyclic AMP-Dependent Protein Kinases
Alanine
Respiration
Proteins
Substitution reactions
Phosphotransferases
Genes
Adenosine Triphosphate

ASJC Scopus subject areas

  • Biochemistry

Cite this

Mitochondrial respiratory mutants in yeast inhibit glycogen accumulation by blocking activation of glycogen synthase. / Yang, Ruojing; Chun, Kristin; Wek, Ronald.

In: Journal of Biological Chemistry, Vol. 273, No. 47, 20.11.1998, p. 31337-31344.

Research output: Contribution to journalArticle

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