Mitotic activity and nucleic acid precursor incorporation in denervated and innervated limb stumps of axolotl larvae.

R. A. Tassava, Anthony Mescher

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Mitotic activity and DNA and RNA precursor incorporation were compared in innervated regenerating limbs and in denervated, non-regenerating limbs on days 8 and 9 post-amputation. Innervated limbs had well-developed cone stage blastemas which showed high cellular mitotic indices and H3-thymidine labeling indices of 0.40-0.50 and H3-uridine labeling indices of 0.50-0.75. In contrast, denervated limbs showed dedifferentiated cells distally under thickened wound epithelia, but essentially no mitotic activity and no blastema formation. These dedifferentiated cells showed lower levels of H3-thymidine (0.10 index) and H3-uridine (0.50) incorporation than regenerating limbs. Labeling indices of wound epithelia are also compared.

Original languageEnglish
Pages (from-to)253-262
Number of pages10
JournalJournal of Experimental Zoology
Volume195
Issue number2
StatePublished - Feb 1976
Externally publishedYes

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stumps
limbs (animal)
nucleic acids
larvae
uridine
thymidine
animal injuries
epithelium
amputation
cells
DNA

ASJC Scopus subject areas

  • Animal Science and Zoology

Cite this

Mitotic activity and nucleic acid precursor incorporation in denervated and innervated limb stumps of axolotl larvae. / Tassava, R. A.; Mescher, Anthony.

In: Journal of Experimental Zoology, Vol. 195, No. 2, 02.1976, p. 253-262.

Research output: Contribution to journalArticle

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