Mitotic activity and nucleic acid precursor incorporation in denervated and innervated limb stumps of axolotl larvae

Roy A. Tassava, Anthony L. Mescher

Research output: Contribution to journalArticle

17 Scopus citations


Mitotic activity and DNA and RNA precursor incorporation were compared in innervated regenerating limbs and in denervated, non-regenerating limbs on days 8 and 9 post-amputation. Innervated limbs had well-developed cone stage blastemas which showed high cellular mitotic indices and H3-thymidine labeling indices of 0.40-0.50 and H3-uridine labeling indices of 0.50-0.75. In contrast, denervated limbs showed dedifferentiated cells distally under thickened wound epithelia, but essentially no mitotic activity and no blastema formation. These dedifferentiated cells showed lower levels of H3-thymidine (0.10 index) and H3-uridine (0.50) incorporation than regenerating limbs. Labeling indices of wound epithelia are also compared.

Original languageEnglish (US)
Pages (from-to)253-262
Number of pages10
JournalJournal of Experimental Zoology
Issue number2
StatePublished - Feb 1976


ASJC Scopus subject areas

  • Animal Science and Zoology

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