Mixed epithelial and stromal tumor of the kidney lacks the genetic alterations of cellular congenital mesoblastic nephroma

Christopher R. Pierson, Megan S. Schober, Tracie Wallis, Fazlul H. Sarkar, Poul H.B. Sorensen, John N. Eble, John R. Srigley, Edward C. Jones, David J. Grignon, Volkan Adsay

Research output: Contribution to journalArticle

45 Scopus citations

Abstract

Mixed epithelial and stromal tumor of the kidney is a recently recognized neoplasm that occurs almost exclusively in perimenopausal women. Because it frequently contains areas of smooth muscle in which epithelial structures are embedded, some have concluded that it is the adult form of congenital mesoblastic nephroma. Others have concluded that the morphology and epidemiology of mixed epithelial and stromal tumor indicate that it is unrelated to congenital mesoblastic nephroma. Although the genetic alterations of mixed epithelial and stromal tumor have not been previously elucidated, much is known about the genetic alterations of cellular congenital mesoblastic nephroma. The present study was undertaken to determine if mixed epithelial and stromal tumors have any of the genetic alterations recognized as typical of cellular congenital mesoblastic nephroma. RNA extraction was performed on formalin-fixed, paraffin-embedded tissue from 7 mixed epithelial and stromal tumors followed by reverse-transcription polymerase chain reaction to detect the ETV6-NTRK3 gene fusion. Fluorescent in situ hybridization with centromere-specific probes for chromosomes 8, 11, and 17 was performed to evaluate polyploidy of these chromosomes in 11 cases of mixed epithelial and stromal tumor. None of the mixed epithelial and stromal tumors showed any of these genetic alterations. We conclude that mixed epithelial and stromal tumor of the kidney lacks the genetic alterations typical of cellular congenital mesoblastic nephroma, is unrelated to it, and the appellation "adult mesoblastic nephroma" should not be used for these tumors.

Original languageEnglish (US)
Pages (from-to)513-520
Number of pages8
JournalHuman pathology
Volume32
Issue number5
DOIs
StatePublished - Jan 1 2001

Keywords

  • Biphasic
  • ETV6-NTRK3 gene fusion
  • Mesoblastic nephroma
  • Mixed tumor
  • Polyploidy
  • Reverse-transcription polymerase chain reaction

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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    Pierson, C. R., Schober, M. S., Wallis, T., Sarkar, F. H., Sorensen, P. H. B., Eble, J. N., Srigley, J. R., Jones, E. C., Grignon, D. J., & Adsay, V. (2001). Mixed epithelial and stromal tumor of the kidney lacks the genetic alterations of cellular congenital mesoblastic nephroma. Human pathology, 32(5), 513-520. https://doi.org/10.1053/hupa.2001.24323