Clones de las células osteocíticas MLO-Y4 tienen diferentes patrones de expresión génica característicos de diferentes estadíos de diferenciación osteocítica

Translated title of the contribution: MLO-Y4 osteocytic cell clones express distinct gene expression patterns characteristic of different stages of osteocyte differentiation

Emily G. Atkinson, Alejandro Marcial, Zuleima Sánchez, Christian Porter, Lilian Plotkin

Research output: Contribution to journalArticle

Abstract

Osteocytes are the most abundant bone cell and are formed when osteoblasts become embedded in the bone matrix. Through changes in gene expression and paracrine effects, osteocytes regulate the number of osteoblasts, bone forming cells, and osteoclasts, bone resorbing cells, which are needed to maintain bone mass. MLO-Y4 is the better characterized osteocytic cell line; however, lacks expression of sclerostin, the product of the SOST gene, which is fundamental for osteocyte function and blocks bone formation. With the objective to isolate MLO-Y4 clones with different gene expression profiles, we performed cultures at very low density of MLO-Y4 cells stably transfected with nuclear green fluorescent protein (MLO-nGFP). Cell morphology was visualized under a fluorescence microscope. Once the cells reached 80% confluency, RNA was extracted and quantitative real time PCR was performed. Clones exhibit different sizes and morphology, with some cells showing a spindle-like shape and others with abundant projections and a star-like shape. Gene expression also differed among clones. However, none of the clones examined expressed SOST. We conclude that the MLO-nGFP clones constitute a useful tool to study osteocyte differentiation and the role of osteocytes in the control of bone formation and resorption in vitro.

Original languageSpanish
Pages (from-to)207-213
Number of pages7
JournalActualizaciones en Osteologia
Volume13
Issue number3
StatePublished - Sep 1 2017

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Osteocytes
Clone Cells
Gene Expression
Bone and Bones
Osteoblasts
Osteogenesis
Bone Matrix
Osteoclasts
Bone Resorption
Nuclear Proteins
Green Fluorescent Proteins
Transcriptome
Real-Time Polymerase Chain Reaction
Fluorescence
RNA
Cell Line
Genes

Keywords

  • Cell morphology
  • Gene expression
  • Osteocytic cells

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Medicine (miscellaneous)
  • Orthopedics and Sports Medicine
  • Radiology Nuclear Medicine and imaging

Cite this

Clones de las células osteocíticas MLO-Y4 tienen diferentes patrones de expresión génica característicos de diferentes estadíos de diferenciación osteocítica. / Atkinson, Emily G.; Marcial, Alejandro; Sánchez, Zuleima; Porter, Christian; Plotkin, Lilian.

In: Actualizaciones en Osteologia, Vol. 13, No. 3, 01.09.2017, p. 207-213.

Research output: Contribution to journalArticle

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