MLR blast cells generated in mutant-standard strain combinations bind H-2K and H-2D antigens

R. W. Geib, J. Klein

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Strains CBA (M523) (=M523) and CBA differ by a mutation which has been mapped genetically into the K region of the H-2 complex. Similarly, strains B10.D2 (M504) (=M504) and B10.D2 differ in a mutation which occurred in the D region. The data presented in this study show that mixed lymphocyte culture in M523 anti-CBA and M504 anti-B10.D2 strain combinations leads to the release of membrane fragments from the stimulating cells and binding of these fragments by blast cells. The fragments always carry H-2K (in the M523 anti-CBA combination) or H-2D (in the M504 anti-B10.D2 combination) antigens present in the stimulating and absent in the responding cells (antigens H-2.60 and H-2.40, respectively). Although Ia antigens may occasionally be present on the CBA membrane fragments, these antigens do not participate in the M523 anti-CBA MLR stimulation. The data thus demonstrte that serologically detectable H-2K and H-2D antigens can induce a MLR and that a mutation can change properties of H-2K or H-2D molecules so that the alteration is detectable by both serological means and lymphocyte activation assays.

Original languageEnglish (US)
Pages (from-to)135-139
Number of pages5
JournalEuropean Journal of Immunology
Volume9
Issue number2
StatePublished - 1979
Externally publishedYes

Fingerprint

Mutation
Antigens
Membranes
Histocompatibility Antigens Class II
Lymphocyte Activation
Lymphocytes
H antigen

ASJC Scopus subject areas

  • Immunology

Cite this

MLR blast cells generated in mutant-standard strain combinations bind H-2K and H-2D antigens. / Geib, R. W.; Klein, J.

In: European Journal of Immunology, Vol. 9, No. 2, 1979, p. 135-139.

Research output: Contribution to journalArticle

@article{469ef7a6c74840e89c25a40736643d2b,
title = "MLR blast cells generated in mutant-standard strain combinations bind H-2K and H-2D antigens",
abstract = "Strains CBA (M523) (=M523) and CBA differ by a mutation which has been mapped genetically into the K region of the H-2 complex. Similarly, strains B10.D2 (M504) (=M504) and B10.D2 differ in a mutation which occurred in the D region. The data presented in this study show that mixed lymphocyte culture in M523 anti-CBA and M504 anti-B10.D2 strain combinations leads to the release of membrane fragments from the stimulating cells and binding of these fragments by blast cells. The fragments always carry H-2K (in the M523 anti-CBA combination) or H-2D (in the M504 anti-B10.D2 combination) antigens present in the stimulating and absent in the responding cells (antigens H-2.60 and H-2.40, respectively). Although Ia antigens may occasionally be present on the CBA membrane fragments, these antigens do not participate in the M523 anti-CBA MLR stimulation. The data thus demonstrte that serologically detectable H-2K and H-2D antigens can induce a MLR and that a mutation can change properties of H-2K or H-2D molecules so that the alteration is detectable by both serological means and lymphocyte activation assays.",
author = "Geib, {R. W.} and J. Klein",
year = "1979",
language = "English (US)",
volume = "9",
pages = "135--139",
journal = "European Journal of Immunology",
issn = "0014-2980",
publisher = "Wiley-VCH Verlag",
number = "2",

}

TY - JOUR

T1 - MLR blast cells generated in mutant-standard strain combinations bind H-2K and H-2D antigens

AU - Geib, R. W.

AU - Klein, J.

PY - 1979

Y1 - 1979

N2 - Strains CBA (M523) (=M523) and CBA differ by a mutation which has been mapped genetically into the K region of the H-2 complex. Similarly, strains B10.D2 (M504) (=M504) and B10.D2 differ in a mutation which occurred in the D region. The data presented in this study show that mixed lymphocyte culture in M523 anti-CBA and M504 anti-B10.D2 strain combinations leads to the release of membrane fragments from the stimulating cells and binding of these fragments by blast cells. The fragments always carry H-2K (in the M523 anti-CBA combination) or H-2D (in the M504 anti-B10.D2 combination) antigens present in the stimulating and absent in the responding cells (antigens H-2.60 and H-2.40, respectively). Although Ia antigens may occasionally be present on the CBA membrane fragments, these antigens do not participate in the M523 anti-CBA MLR stimulation. The data thus demonstrte that serologically detectable H-2K and H-2D antigens can induce a MLR and that a mutation can change properties of H-2K or H-2D molecules so that the alteration is detectable by both serological means and lymphocyte activation assays.

AB - Strains CBA (M523) (=M523) and CBA differ by a mutation which has been mapped genetically into the K region of the H-2 complex. Similarly, strains B10.D2 (M504) (=M504) and B10.D2 differ in a mutation which occurred in the D region. The data presented in this study show that mixed lymphocyte culture in M523 anti-CBA and M504 anti-B10.D2 strain combinations leads to the release of membrane fragments from the stimulating cells and binding of these fragments by blast cells. The fragments always carry H-2K (in the M523 anti-CBA combination) or H-2D (in the M504 anti-B10.D2 combination) antigens present in the stimulating and absent in the responding cells (antigens H-2.60 and H-2.40, respectively). Although Ia antigens may occasionally be present on the CBA membrane fragments, these antigens do not participate in the M523 anti-CBA MLR stimulation. The data thus demonstrte that serologically detectable H-2K and H-2D antigens can induce a MLR and that a mutation can change properties of H-2K or H-2D molecules so that the alteration is detectable by both serological means and lymphocyte activation assays.

UR - http://www.scopus.com/inward/record.url?scp=0018774619&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0018774619&partnerID=8YFLogxK

M3 - Article

VL - 9

SP - 135

EP - 139

JO - European Journal of Immunology

JF - European Journal of Immunology

SN - 0014-2980

IS - 2

ER -