MMP and TIMP temporal gene expression during osteocytogenesis

M. Prideaux, K. A. Staines, E. R. Jones, G. P. Riley, A. A. Pitsillides, C. Farquharson

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Osteocytes within bone differentiate from osteoblast precursors which reside in a mineralised extracellular matrix (ECM). Fully differentiated osteocytes are critical for bone development and function but the factors that regulate this differentiation process are unknown. The enzymes primarily responsible for ECM remodelling are matrix metalloproteinases (MMP); however, the expression and role of MMPs during osteocytogenesis is undefined. Here we used MLO-A5 cells to determine the temporal gene expressions of the MMP family and their endogenous inhibitors - tissue inhibitors of metalloproteinases (TIMPs) during osteocytogenesis. RT-qPCR revealed expression of 14 Mmps and 3 Timps in MLO-A5 cells. Mmp2, Mmp23 and Mmp28 were decreased concurrent with mineralisation onset (P < 0.05∗). Mmp14 and Mmp19 mRNAs were also significantly increased at day 3 (P < 0.05∗) before returning to baseline levels at day 6. Decreased expressions of Timp1, Timp2 and Timp3 mRNA were observed by day 6 compared to day 0 (P < 0.05∗). To examine whether these changes are linked to osteocytogenesis, we determined Mmp/Timp mRNA expressions in mineralisation-limited conditions. RT-qPCR revealed that the previously observed decreases in Mmp2, Mmp23 and Mmp28 were not observed in these mineralisation-limited cultures, therefore closely linking these MMPs with osteocyte differentiation. Similarly, we found differential expression of Timp1, Timp2 and Timp3 mRNA in mineralisation-restricted cultures (P< 0.05∗). In conclusion, we have identified several members of the MMP/TIMP families as regulators of ECM remodelling necessary for the acquisition of the osteocyte phenotype.

Original languageEnglish (US)
Pages (from-to)29-36
Number of pages8
JournalGene Expression Patterns
Volume18
Issue number1-2
DOIs
StatePublished - May 28 2015
Externally publishedYes

Fingerprint

Tissue Inhibitor of Metalloproteinases
Osteocytes
Matrix Metalloproteinase Inhibitors
Matrix Metalloproteinases
Extracellular Matrix
Gene Expression
Messenger RNA
Bone Development
Osteoblasts
Phenotype
Bone and Bones
Enzymes

Keywords

  • Mineralisation
  • MMP
  • Osteoblast
  • Osteocyte
  • TIMP

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Developmental Biology

Cite this

Prideaux, M., Staines, K. A., Jones, E. R., Riley, G. P., Pitsillides, A. A., & Farquharson, C. (2015). MMP and TIMP temporal gene expression during osteocytogenesis. Gene Expression Patterns, 18(1-2), 29-36. https://doi.org/10.1016/j.gep.2015.04.004

MMP and TIMP temporal gene expression during osteocytogenesis. / Prideaux, M.; Staines, K. A.; Jones, E. R.; Riley, G. P.; Pitsillides, A. A.; Farquharson, C.

In: Gene Expression Patterns, Vol. 18, No. 1-2, 28.05.2015, p. 29-36.

Research output: Contribution to journalArticle

Prideaux, M, Staines, KA, Jones, ER, Riley, GP, Pitsillides, AA & Farquharson, C 2015, 'MMP and TIMP temporal gene expression during osteocytogenesis', Gene Expression Patterns, vol. 18, no. 1-2, pp. 29-36. https://doi.org/10.1016/j.gep.2015.04.004
Prideaux M, Staines KA, Jones ER, Riley GP, Pitsillides AA, Farquharson C. MMP and TIMP temporal gene expression during osteocytogenesis. Gene Expression Patterns. 2015 May 28;18(1-2):29-36. https://doi.org/10.1016/j.gep.2015.04.004
Prideaux, M. ; Staines, K. A. ; Jones, E. R. ; Riley, G. P. ; Pitsillides, A. A. ; Farquharson, C. / MMP and TIMP temporal gene expression during osteocytogenesis. In: Gene Expression Patterns. 2015 ; Vol. 18, No. 1-2. pp. 29-36.
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