Mobilization and collection of peripheral blood CD34 + cells from patients with Fanconi anemia

James Croop, Ryan Cooper, Christine Fernandez, Vicki Graves, Susan Kreissman, Helmut Hanenberg, Franklin O. Smith, David A. Williams

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

A potential therapeutic option for patients with Fanconi anemia is collection of peripheral blood stem cells prior to the development of severe pancytopenia. These hematopoietic cells potentially could be infused when symptomatic bone marrow failure develops, as autologous rescue after chemotherapy in the event of leukemic transformation, or as targets for gene therapy. Eight patients with Fanconi anemia were mobilized with 10 μg/kg per day of granulocyte colony-stimulating factor (median, 10 ± 4 days) to determine the feasibility of collecting peripheral blood stem cells for future use. Six patients achieved a peripheral blood CD34 + count of ≥6/μL and underwent apheresis. The collection goal was 2 × 10 6 CD34 + cells/kg based on a predicted weight 5 years from the date of collection. A mean of 2.6 ± 0.9 × 10 6 CD34 + cells/kg of the weight at the time of collection were collected, which corresponded to 1.9 ± 0.4 × 10 6 CD34 + cells/kg of the target weight. The collections required a mean of 4 ± 3 days (range, 2-8 days) of apheresis. Six of the 8 subjects had ≥ 1 × 10 6 CD34 + cells/kg cryopreserved based on both actual and target weights, and 4 subjects had ≥ 2 × 10 6 CD34 + cells/kg cryopreserved based on the target weight. These results suggest that some patients with Fanconi anemia can have adequate numbers of CD34 + cells mobilized and collected from the peripheral blood prior to the onset of severe bone marrow failure, but they may require an extended mobilization and multiple days of collection.

Original languageEnglish
Pages (from-to)2917-2921
Number of pages5
JournalBlood
Volume98
Issue number10
DOIs
StatePublished - Nov 15 2001

Fingerprint

Fanconi Anemia
Blood Cells
Blood
Weights and Measures
Stem cells
Bone
Blood Component Removal
Gene therapy
Chemotherapy
Granulocyte Colony-Stimulating Factor
Bone Marrow
Pancytopenia
Genetic Therapy
Cell Count
Drug Therapy

ASJC Scopus subject areas

  • Hematology

Cite this

Croop, J., Cooper, R., Fernandez, C., Graves, V., Kreissman, S., Hanenberg, H., ... Williams, D. A. (2001). Mobilization and collection of peripheral blood CD34 + cells from patients with Fanconi anemia. Blood, 98(10), 2917-2921. https://doi.org/10.1182/blood.V98.10.2917

Mobilization and collection of peripheral blood CD34 + cells from patients with Fanconi anemia. / Croop, James; Cooper, Ryan; Fernandez, Christine; Graves, Vicki; Kreissman, Susan; Hanenberg, Helmut; Smith, Franklin O.; Williams, David A.

In: Blood, Vol. 98, No. 10, 15.11.2001, p. 2917-2921.

Research output: Contribution to journalArticle

Croop, J, Cooper, R, Fernandez, C, Graves, V, Kreissman, S, Hanenberg, H, Smith, FO & Williams, DA 2001, 'Mobilization and collection of peripheral blood CD34 + cells from patients with Fanconi anemia', Blood, vol. 98, no. 10, pp. 2917-2921. https://doi.org/10.1182/blood.V98.10.2917
Croop J, Cooper R, Fernandez C, Graves V, Kreissman S, Hanenberg H et al. Mobilization and collection of peripheral blood CD34 + cells from patients with Fanconi anemia. Blood. 2001 Nov 15;98(10):2917-2921. https://doi.org/10.1182/blood.V98.10.2917
Croop, James ; Cooper, Ryan ; Fernandez, Christine ; Graves, Vicki ; Kreissman, Susan ; Hanenberg, Helmut ; Smith, Franklin O. ; Williams, David A. / Mobilization and collection of peripheral blood CD34 + cells from patients with Fanconi anemia. In: Blood. 2001 ; Vol. 98, No. 10. pp. 2917-2921.
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