Modeling early retinal development with human embryonic and induced pluripotent stem cells

Jason S. Meyer, Rebecca L. Shearer, Elizabeth E. Capowski, Lynda S. Wright, Kyle A. Wallace, Erin L. McMillan, Su Chun Zhang, David M. Gamm

Research output: Contribution to journalArticlepeer-review

386 Scopus citations

Abstract

Human pluripotent stem cells have the potential to provide comprehensive model systems for the earliest stages of human ontogenesis. To serve in this capacity, these cells must undergo a targeted, stepwise differentiation process that follows a normal developmental timeline. Here we demonstrate the ability of both human embryonic stem cells (hESCs) and induced pluripotent stem (iPS) cells to meet these requirements for human retinogenesis. Upon differentiation, hESCs initially yielded a highly enriched population of early eye field cells. Thereafter, a subset of cells acquired features of advancing retinal differentiation in a sequence and time course that mimicked in vivo human retinal development. Application of this culture method to a human iPS cell line also generated retina-specific cell types at comparable times in vitro. Lastly, altering endogenous signaling during differentiation affected lineage-specific gene expression in a manner consistent with established mechanisms of early neural and retinal cell fate determination. These findings should aid in the investigation of the molecular events governing retinal specification from human pluripotent stem cells.

Original languageEnglish (US)
Pages (from-to)16698-16703
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume106
Issue number39
DOIs
StatePublished - Sep 29 2009

ASJC Scopus subject areas

  • General

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