Modification by clodronate and fluoride of hydroxyapatite-induced neutrophil chemiluminescence in vitro.

P. M. Hyvönen, Michael Kowolik

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

In the light of previous studies demonstrating an inhibition of oxidative metabolism in human neutrophil granulocytes by the bisphosphonate, clodronate, and considerable evidence of oxidative pathway stimulation by fluoride, the aim of this study was to determine the combined effect of these two agents. A luminol-dependent, fMLP stimulated chemiluminescence system was used and showed that 10(-3) M fluoride significantly increased the inhibitory effect of clodronate (3 ug/ml and 10 ug/ml) when bound to particulate hydroxyapatite (HA), phagocytosed by the neutrophils. By contrast, 10(-4) M fluoride appeared to counteract this inhibition and was subsequently shown, following more prolonged incubation within the cell model system, to significantly enhance cellular oxidative activity by 25% compared to the HA/clodronate control. It is likely that the kinetics of clodronate and fluoride follow a different time course and their intracellular target receptors differ, but the cellular model employed may help to elucidate more precisely the basis of their therapeutic use in bone diseases.

Original languageEnglish (US)
Pages (from-to)63-72
Number of pages10
JournalJournal of Clinical and Laboratory Immunology
Volume46
Issue number2
StatePublished - 1995
Externally publishedYes

Fingerprint

Clodronic Acid
Durapatite
Luminescence
Fluorides
Neutrophils
Luminol
Bone Diseases
Diphosphonates
Therapeutic Uses
Phagocytosis
Granulocytes
In Vitro Techniques

ASJC Scopus subject areas

  • Immunology

Cite this

@article{9f2236089acf4cddaa854377ceedf34c,
title = "Modification by clodronate and fluoride of hydroxyapatite-induced neutrophil chemiluminescence in vitro.",
abstract = "In the light of previous studies demonstrating an inhibition of oxidative metabolism in human neutrophil granulocytes by the bisphosphonate, clodronate, and considerable evidence of oxidative pathway stimulation by fluoride, the aim of this study was to determine the combined effect of these two agents. A luminol-dependent, fMLP stimulated chemiluminescence system was used and showed that 10(-3) M fluoride significantly increased the inhibitory effect of clodronate (3 ug/ml and 10 ug/ml) when bound to particulate hydroxyapatite (HA), phagocytosed by the neutrophils. By contrast, 10(-4) M fluoride appeared to counteract this inhibition and was subsequently shown, following more prolonged incubation within the cell model system, to significantly enhance cellular oxidative activity by 25{\%} compared to the HA/clodronate control. It is likely that the kinetics of clodronate and fluoride follow a different time course and their intracellular target receptors differ, but the cellular model employed may help to elucidate more precisely the basis of their therapeutic use in bone diseases.",
author = "Hyv{\"o}nen, {P. M.} and Michael Kowolik",
year = "1995",
language = "English (US)",
volume = "46",
pages = "63--72",
journal = "Journal of Clinical and Laboratory Immunology",
issn = "0141-2760",
publisher = "Teviot Scientific Publications Ltd.",
number = "2",

}

TY - JOUR

T1 - Modification by clodronate and fluoride of hydroxyapatite-induced neutrophil chemiluminescence in vitro.

AU - Hyvönen, P. M.

AU - Kowolik, Michael

PY - 1995

Y1 - 1995

N2 - In the light of previous studies demonstrating an inhibition of oxidative metabolism in human neutrophil granulocytes by the bisphosphonate, clodronate, and considerable evidence of oxidative pathway stimulation by fluoride, the aim of this study was to determine the combined effect of these two agents. A luminol-dependent, fMLP stimulated chemiluminescence system was used and showed that 10(-3) M fluoride significantly increased the inhibitory effect of clodronate (3 ug/ml and 10 ug/ml) when bound to particulate hydroxyapatite (HA), phagocytosed by the neutrophils. By contrast, 10(-4) M fluoride appeared to counteract this inhibition and was subsequently shown, following more prolonged incubation within the cell model system, to significantly enhance cellular oxidative activity by 25% compared to the HA/clodronate control. It is likely that the kinetics of clodronate and fluoride follow a different time course and their intracellular target receptors differ, but the cellular model employed may help to elucidate more precisely the basis of their therapeutic use in bone diseases.

AB - In the light of previous studies demonstrating an inhibition of oxidative metabolism in human neutrophil granulocytes by the bisphosphonate, clodronate, and considerable evidence of oxidative pathway stimulation by fluoride, the aim of this study was to determine the combined effect of these two agents. A luminol-dependent, fMLP stimulated chemiluminescence system was used and showed that 10(-3) M fluoride significantly increased the inhibitory effect of clodronate (3 ug/ml and 10 ug/ml) when bound to particulate hydroxyapatite (HA), phagocytosed by the neutrophils. By contrast, 10(-4) M fluoride appeared to counteract this inhibition and was subsequently shown, following more prolonged incubation within the cell model system, to significantly enhance cellular oxidative activity by 25% compared to the HA/clodronate control. It is likely that the kinetics of clodronate and fluoride follow a different time course and their intracellular target receptors differ, but the cellular model employed may help to elucidate more precisely the basis of their therapeutic use in bone diseases.

UR - http://www.scopus.com/inward/record.url?scp=0029440275&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029440275&partnerID=8YFLogxK

M3 - Article

VL - 46

SP - 63

EP - 72

JO - Journal of Clinical and Laboratory Immunology

JF - Journal of Clinical and Laboratory Immunology

SN - 0141-2760

IS - 2

ER -