Abstract
Human cells of glial origin were examined for clonogenic survival after exposure to subnormal pH of the extracellular fluid for 0 to 5 days at 37°C. The surviving fraction decreased at pH values between 6.7 and 6.4 to 10% within 2 days. Cells that survived irradiation demonstrated a decreased sensitivity to an acid extracellular fluid. The radiation response function was established with and without incubation at pH 6.7 for 66 hours. Doses above 4 Gray resulted in a higher survival at pH 6.7 than at pH 7.4, if pH was reduced 15 hours prior to irradiation. Enhanced clonogenic survival after irradiation under conditions of continuous acidity in the extracellular space may be a further explanation for poor local control of malignant tumors in clinical radiation therapy.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1659-1662 |
| Number of pages | 4 |
| Journal | International Journal of Radiation Oncology Biology Physics |
| Volume | 6 |
| Issue number | 12 |
| DOIs | |
| State | Published - 1980 |
| Externally published | Yes |
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Keywords
- Extracellular milieu
- Glioblastoma cells (human)
- Hydrogen ion concentration
- Low dose rate
- pH
- Radiosensitivity
ASJC Scopus subject areas
- Oncology
- Radiology Nuclear Medicine and imaging
- Radiation
Cite this
Modification of pH induced cellular inactivation by irradiation-glial cells. / Röttinger, Erwin M.; Mendonca, Marc; Gerweck, Leo E.
In: International Journal of Radiation Oncology Biology Physics, Vol. 6, No. 12, 1980, p. 1659-1662.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Modification of pH induced cellular inactivation by irradiation-glial cells
AU - Röttinger, Erwin M.
AU - Mendonca, Marc
AU - Gerweck, Leo E.
PY - 1980
Y1 - 1980
N2 - Human cells of glial origin were examined for clonogenic survival after exposure to subnormal pH of the extracellular fluid for 0 to 5 days at 37°C. The surviving fraction decreased at pH values between 6.7 and 6.4 to 10% within 2 days. Cells that survived irradiation demonstrated a decreased sensitivity to an acid extracellular fluid. The radiation response function was established with and without incubation at pH 6.7 for 66 hours. Doses above 4 Gray resulted in a higher survival at pH 6.7 than at pH 7.4, if pH was reduced 15 hours prior to irradiation. Enhanced clonogenic survival after irradiation under conditions of continuous acidity in the extracellular space may be a further explanation for poor local control of malignant tumors in clinical radiation therapy.
AB - Human cells of glial origin were examined for clonogenic survival after exposure to subnormal pH of the extracellular fluid for 0 to 5 days at 37°C. The surviving fraction decreased at pH values between 6.7 and 6.4 to 10% within 2 days. Cells that survived irradiation demonstrated a decreased sensitivity to an acid extracellular fluid. The radiation response function was established with and without incubation at pH 6.7 for 66 hours. Doses above 4 Gray resulted in a higher survival at pH 6.7 than at pH 7.4, if pH was reduced 15 hours prior to irradiation. Enhanced clonogenic survival after irradiation under conditions of continuous acidity in the extracellular space may be a further explanation for poor local control of malignant tumors in clinical radiation therapy.
KW - Extracellular milieu
KW - Glioblastoma cells (human)
KW - Hydrogen ion concentration
KW - Low dose rate
KW - pH
KW - Radiosensitivity
UR - http://www.scopus.com/inward/record.url?scp=0019275826&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0019275826&partnerID=8YFLogxK
U2 - 10.1016/0360-3016(80)90248-5
DO - 10.1016/0360-3016(80)90248-5
M3 - Article
C2 - 6263832
AN - SCOPUS:0019275826
VL - 6
SP - 1659
EP - 1662
JO - International Journal of Radiation Oncology Biology Physics
JF - International Journal of Radiation Oncology Biology Physics
SN - 0360-3016
IS - 12
ER -