Modification of pH induced cellular inactivation by irradiation-glial cells

Erwin M. Röttinger, Marc Mendonca, Leo E. Gerweck

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Human cells of glial origin were examined for clonogenic survival after exposure to subnormal pH of the extracellular fluid for 0 to 5 days at 37°C. The surviving fraction decreased at pH values between 6.7 and 6.4 to 10% within 2 days. Cells that survived irradiation demonstrated a decreased sensitivity to an acid extracellular fluid. The radiation response function was established with and without incubation at pH 6.7 for 66 hours. Doses above 4 Gray resulted in a higher survival at pH 6.7 than at pH 7.4, if pH was reduced 15 hours prior to irradiation. Enhanced clonogenic survival after irradiation under conditions of continuous acidity in the extracellular space may be a further explanation for poor local control of malignant tumors in clinical radiation therapy.

Original languageEnglish (US)
Pages (from-to)1659-1662
Number of pages4
JournalInternational Journal of Radiation Oncology Biology Physics
Volume6
Issue number12
DOIs
StatePublished - 1980
Externally publishedYes

Fingerprint

Neuroglia
deactivation
irradiation
Extracellular Fluid
Survival
fluids
Extracellular Space
acidity
radiation therapy
Radiotherapy
tumors
Radiation
dosage
acids
Acids
sensitivity
radiation
cells
Neoplasms

Keywords

  • Extracellular milieu
  • Glioblastoma cells (human)
  • Hydrogen ion concentration
  • Low dose rate
  • pH
  • Radiosensitivity

ASJC Scopus subject areas

  • Oncology
  • Radiology Nuclear Medicine and imaging
  • Radiation

Cite this

Modification of pH induced cellular inactivation by irradiation-glial cells. / Röttinger, Erwin M.; Mendonca, Marc; Gerweck, Leo E.

In: International Journal of Radiation Oncology Biology Physics, Vol. 6, No. 12, 1980, p. 1659-1662.

Research output: Contribution to journalArticle

@article{32860a809d8e4dce82dc826b748a47a5,
title = "Modification of pH induced cellular inactivation by irradiation-glial cells",
abstract = "Human cells of glial origin were examined for clonogenic survival after exposure to subnormal pH of the extracellular fluid for 0 to 5 days at 37°C. The surviving fraction decreased at pH values between 6.7 and 6.4 to 10{\%} within 2 days. Cells that survived irradiation demonstrated a decreased sensitivity to an acid extracellular fluid. The radiation response function was established with and without incubation at pH 6.7 for 66 hours. Doses above 4 Gray resulted in a higher survival at pH 6.7 than at pH 7.4, if pH was reduced 15 hours prior to irradiation. Enhanced clonogenic survival after irradiation under conditions of continuous acidity in the extracellular space may be a further explanation for poor local control of malignant tumors in clinical radiation therapy.",
keywords = "Extracellular milieu, Glioblastoma cells (human), Hydrogen ion concentration, Low dose rate, pH, Radiosensitivity",
author = "R{\"o}ttinger, {Erwin M.} and Marc Mendonca and Gerweck, {Leo E.}",
year = "1980",
doi = "10.1016/0360-3016(80)90248-5",
language = "English (US)",
volume = "6",
pages = "1659--1662",
journal = "International Journal of Radiation Oncology Biology Physics",
issn = "0360-3016",
publisher = "Elsevier Inc.",
number = "12",

}

TY - JOUR

T1 - Modification of pH induced cellular inactivation by irradiation-glial cells

AU - Röttinger, Erwin M.

AU - Mendonca, Marc

AU - Gerweck, Leo E.

PY - 1980

Y1 - 1980

N2 - Human cells of glial origin were examined for clonogenic survival after exposure to subnormal pH of the extracellular fluid for 0 to 5 days at 37°C. The surviving fraction decreased at pH values between 6.7 and 6.4 to 10% within 2 days. Cells that survived irradiation demonstrated a decreased sensitivity to an acid extracellular fluid. The radiation response function was established with and without incubation at pH 6.7 for 66 hours. Doses above 4 Gray resulted in a higher survival at pH 6.7 than at pH 7.4, if pH was reduced 15 hours prior to irradiation. Enhanced clonogenic survival after irradiation under conditions of continuous acidity in the extracellular space may be a further explanation for poor local control of malignant tumors in clinical radiation therapy.

AB - Human cells of glial origin were examined for clonogenic survival after exposure to subnormal pH of the extracellular fluid for 0 to 5 days at 37°C. The surviving fraction decreased at pH values between 6.7 and 6.4 to 10% within 2 days. Cells that survived irradiation demonstrated a decreased sensitivity to an acid extracellular fluid. The radiation response function was established with and without incubation at pH 6.7 for 66 hours. Doses above 4 Gray resulted in a higher survival at pH 6.7 than at pH 7.4, if pH was reduced 15 hours prior to irradiation. Enhanced clonogenic survival after irradiation under conditions of continuous acidity in the extracellular space may be a further explanation for poor local control of malignant tumors in clinical radiation therapy.

KW - Extracellular milieu

KW - Glioblastoma cells (human)

KW - Hydrogen ion concentration

KW - Low dose rate

KW - pH

KW - Radiosensitivity

UR - http://www.scopus.com/inward/record.url?scp=0019275826&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0019275826&partnerID=8YFLogxK

U2 - 10.1016/0360-3016(80)90248-5

DO - 10.1016/0360-3016(80)90248-5

M3 - Article

C2 - 6263832

AN - SCOPUS:0019275826

VL - 6

SP - 1659

EP - 1662

JO - International Journal of Radiation Oncology Biology Physics

JF - International Journal of Radiation Oncology Biology Physics

SN - 0360-3016

IS - 12

ER -