Modification of yeast Cdc53p by the ubiquitin-related protein Rub1p affects function of the SCF(Cdc4) complex

Doug Lammer, Neal Mathias, Jose M. Laplaza, Weidong Jiang, Yun Liu, Judy Callis, Mark Goebl, Mark Estelle

Research output: Contribution to journalArticle

268 Citations (Scopus)

Abstract

The RUB1/NEDD-8 family of ubiquitin-related genes is widely represented among eukaryotes. Here we report that Cdc53p in Saccharomyces cerevisiae, a member of the Cullin family of proteins, is stably modified by the covalent attachment of a single Rub1p molecule. Two genes have been identified that are required for Rub1p conjugation to Cdc53p. The first gene, designated ENR2, encodes a protein with sequence similarity to the amino-terminal half of the ubiquitin-activating enzyme. By analogy with Aos1p, we infer that Enr2p functions in a bipartite Rub1p-activating enzyme. The second gene is SKP1, shown previously to be required for some ubiquitin-conjugation events. A deletion allele of ENR2 is lethal with temperature-sensitive alleles of cdc34 and enhances the phenotypes of cdc4, cdc55, and skp1, strongly implying that Rub1p conjugation to Cdc53p is required for optimal assembly or function of the E3 complex SCF(Cdc4). Consistent with this model, both enr2Δ and an allele of Cdc53p that is not Rub1p modified, render cells sensitive to alterations in the levels of Cdc4p, Cdc34p, and Cdc53p.

Original languageEnglish
Pages (from-to)914-926
Number of pages13
JournalGenes and Development
Volume12
Issue number7
StatePublished - Apr 1 1998

Fingerprint

Ubiquitin
Yeasts
Alleles
Genes
Proteins
Ubiquitin-Activating Enzymes
Cullin Proteins
Eukaryota
Saccharomyces cerevisiae
Phenotype
Temperature
Enzymes

Keywords

  • Auxin
  • Cdc53p
  • Rub1p molecule
  • SCF(Cdc34)
  • Ubiquitin
  • Yeast

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology

Cite this

Lammer, D., Mathias, N., Laplaza, J. M., Jiang, W., Liu, Y., Callis, J., ... Estelle, M. (1998). Modification of yeast Cdc53p by the ubiquitin-related protein Rub1p affects function of the SCF(Cdc4) complex. Genes and Development, 12(7), 914-926.

Modification of yeast Cdc53p by the ubiquitin-related protein Rub1p affects function of the SCF(Cdc4) complex. / Lammer, Doug; Mathias, Neal; Laplaza, Jose M.; Jiang, Weidong; Liu, Yun; Callis, Judy; Goebl, Mark; Estelle, Mark.

In: Genes and Development, Vol. 12, No. 7, 01.04.1998, p. 914-926.

Research output: Contribution to journalArticle

Lammer, D, Mathias, N, Laplaza, JM, Jiang, W, Liu, Y, Callis, J, Goebl, M & Estelle, M 1998, 'Modification of yeast Cdc53p by the ubiquitin-related protein Rub1p affects function of the SCF(Cdc4) complex', Genes and Development, vol. 12, no. 7, pp. 914-926.
Lammer D, Mathias N, Laplaza JM, Jiang W, Liu Y, Callis J et al. Modification of yeast Cdc53p by the ubiquitin-related protein Rub1p affects function of the SCF(Cdc4) complex. Genes and Development. 1998 Apr 1;12(7):914-926.
Lammer, Doug ; Mathias, Neal ; Laplaza, Jose M. ; Jiang, Weidong ; Liu, Yun ; Callis, Judy ; Goebl, Mark ; Estelle, Mark. / Modification of yeast Cdc53p by the ubiquitin-related protein Rub1p affects function of the SCF(Cdc4) complex. In: Genes and Development. 1998 ; Vol. 12, No. 7. pp. 914-926.
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