Modular nanoparticle probes for personalized in vitro and in vivo imaging of cancer cell populations

P. D. Nallathamby, K. Cowden-Dahl, R. K. Roeder

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Contrast-enhanced computed tomography (CT) and spectral (color) X-ray CT have the potential to enable molecular imaging in CT and complement PET scans and MRI scans as a cheaper yet highly sensitive, high resolution, diagnostic tool. However, there is a lack of contrast agents designed to leverage the capabilities of spectral CT fully. In this talk, we will present a modular approach we have developed to create a spectral library of core-shell nanoparticle (NPs) contrast agents. The synthesis method will have broad applications in biomedical imaging due to potential for scalable synthesis of multi-modal imaging probes (e.g., fluorescence, MRI, X-ray, plasmonic resonance), dosed delivery of therapeutics and active targeting through molecular surface functionalization. Gold NPs (Au NPs), gadolinium oxide NPs (Gd203 NPs) and hafnium oxide NPs (Hf02 NPs) core compositions were prepared at a standard size (-10 nm) using solution phase synthesis. Controlled addition of silica shells (1-15 nm) enabled controlled loading of fluorescent molecules and provided a common platform for molecular surface functionalization. Antibodies and other small molecules were efficiently conjugated to the nanoparticles using appropriate CLICK chemistry. The bioactivity and orientation of antibodies conjugated to NPs were confirmed through agglomeration assays and electron microscopy. Quantitative assays reported a steady conjugation efficiency of 75%-80%. Bioconjugation allowed the multi-modal bioactive NPs were then successfully targeted in vitro and in vivo to metastasis-promoting cdl33(+) SKOV3-IP cells, which are responsible for poor prognosis in ovarian cancer patients. The in-vitro and in-vivo data can be discussed in detail with the corresponding author.

Original languageEnglish (US)
Title of host publicationBiotech, Biomaterials and Biomedical - TechConnect Briefs 2017
EditorsFiona Case, Matthew Laudon, Fiona Case, Bart Romanowicz
PublisherTechConnect
Pages122-125
Number of pages4
ISBN (Electronic)9780998878201
StatePublished - Jan 1 2017
Event11th Annual TechConnect World Innovation Conference and Expo, Held Jointly with the 20th Annual Nanotech Conference and Expo, and the 2017 National SBIR/STTR Conference - Washington, United States
Duration: May 14 2017May 17 2017

Publication series

NameAdvanced Materials - TechConnect Briefs 2017
Volume3

Other

Other11th Annual TechConnect World Innovation Conference and Expo, Held Jointly with the 20th Annual Nanotech Conference and Expo, and the 2017 National SBIR/STTR Conference
CountryUnited States
CityWashington
Period5/14/175/17/17

Keywords

  • Core-shell
  • Modular platform
  • Ovarian cancer
  • Spectral CT
  • X-ray CT

ASJC Scopus subject areas

  • Fuel Technology
  • Surfaces, Coatings and Films
  • Biotechnology
  • Fluid Flow and Transfer Processes

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  • Cite this

    Nallathamby, P. D., Cowden-Dahl, K., & Roeder, R. K. (2017). Modular nanoparticle probes for personalized in vitro and in vivo imaging of cancer cell populations. In F. Case, M. Laudon, F. Case, & B. Romanowicz (Eds.), Biotech, Biomaterials and Biomedical - TechConnect Briefs 2017 (pp. 122-125). (Advanced Materials - TechConnect Briefs 2017; Vol. 3). TechConnect.