Modulation of calcium uptake in cultured cardiac muscle cells by 1,25-dihydroxyvitamin D3

Juana Sellés, Teresita Bellido, Ricardo Boland

Research output: Contribution to journalArticle

29 Scopus citations

Abstract

Recent studies have provided evidence indicating that 1,25-Dihydroxyvitamin D3 [1,25(OH)2D3] rapidly stimulates calcium influx through Ca2+ channels in isolated chick heart tissue and cells. Studies were performed both to evaluate the characteristics of the hormone action in cultured myocytes and to obtain information on the metabolic pathway which mediates its effects. Treatment of 70-80% confluent chick embryo myocyte monolayers with 1,25(OH)2D3 induced a fast (within 3-5 min) stimulation of 45Ca uptake which was dose-dependent, maximum responses (130% above controls) being elicited at a concentration of 10-10M. Physiological levels of 25(OH)D3 and 24,25(OH)2D3 and the synthetic analog 1α(OH)D3, had lower activity. Coincident with the rapid changes in Ca uptake, 1,25(OH)2D3 significantly increased cAMP levels. The hormone-induced increase in cAMP was not blocked by nifedipine. Compound SQ 22536, a specific inhibitor of adenylate cyclase, completely suppressed the effects of the sterol on cAMP and Ca uptake. Furthermore, GDP-β-S inhibited the increase in Ca uptake by 1,25(OH)2D3. These results involve the adenylate cyclase pathway and the participation of G proteins in 1,25(OH)2D3 stimulation of Ca influx in chick heart cells.

Original languageEnglish (US)
Pages (from-to)1593-1599
Number of pages7
JournalJournal of Molecular and Cellular Cardiology
Volume26
Issue number12
DOIs
StatePublished - Dec 1994
Externally publishedYes

Keywords

  • 1,25-Dihydroxy vitamin D
  • Ca uptake
  • Myocytes

ASJC Scopus subject areas

  • Molecular Biology
  • Cardiology and Cardiovascular Medicine

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