Modulation of chloride secretion in the rat colon by intracellular bicarbonate

Pierre Dagher, Leah Balsam, J. Todd Weber, Richard W. Egnor, Alan N. Charney

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Extracellular HCO3- stimulates colonic net Cl- absorption in part by inhibiting basal Cl- secretion. This inhibition was investigated by measuring serosal-to-mucosal Cl- flux across short-circuited colonic segments from Sprague-Dawley rats. Mucosal intracellular pH and bicarbonate were estimated using the pH-sensitive dye BCECF. When extracellular [HCO3post-] ([HCO3post-]e) was increased from 0 to 39 mmol/L at Pco2 33 mm Hg, mucosal intracellular [HCO3post-] ([HCO3post-]i) increased to 25.3 mmol/L and serosal-to-mucosal Cl- flux decreased from 13.0 to 7.1 μEq · cm-2 · h-1. When Pco2 was increased to 72 mm Hg at [HCO3post-]e 39 mmol/L, [HCO3post-]i increased to 29.8 mmol/L and serosal-to-mucosal Cl- flux decreased to 5.9 μEq · cm-2 · h-1. In Ringer's solution containing 21 mmol/L HCO3post- and 20 mmol/L Cl- (but not 100 mmol/L Cl-), increasing Pco2 from 21 to 70 mm Hg increased [HCO3post-]i to 22.6 mmol/L and decreased serosal-to-mucosal Cl- flux from 3.0 to 1.7 μEq · cm-2 · h-1. Overall, serosal-to-mucosal Cl- flux was inversely related to [HCO3post-]i on either side of an [HCO3post-]i plateau of 9-18 mmol/L at which flux was stable. These data suggest that [HCO3post-]i is an important modulator of basal Cl- secretion in rat distal colon.

Original languageEnglish (US)
Pages (from-to)120-127
Number of pages8
JournalGastroenterology
Volume103
Issue number1
StatePublished - 1992
Externally publishedYes

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Bicarbonates
Chlorides
Colon
Sprague Dawley Rats
Coloring Agents
2',7'-bis(carboxyethyl)-5(6)-carboxyfluorescein
Ringer's solution

ASJC Scopus subject areas

  • Gastroenterology

Cite this

Dagher, P., Balsam, L., Weber, J. T., Egnor, R. W., & Charney, A. N. (1992). Modulation of chloride secretion in the rat colon by intracellular bicarbonate. Gastroenterology, 103(1), 120-127.

Modulation of chloride secretion in the rat colon by intracellular bicarbonate. / Dagher, Pierre; Balsam, Leah; Weber, J. Todd; Egnor, Richard W.; Charney, Alan N.

In: Gastroenterology, Vol. 103, No. 1, 1992, p. 120-127.

Research output: Contribution to journalArticle

Dagher, P, Balsam, L, Weber, JT, Egnor, RW & Charney, AN 1992, 'Modulation of chloride secretion in the rat colon by intracellular bicarbonate', Gastroenterology, vol. 103, no. 1, pp. 120-127.
Dagher P, Balsam L, Weber JT, Egnor RW, Charney AN. Modulation of chloride secretion in the rat colon by intracellular bicarbonate. Gastroenterology. 1992;103(1):120-127.
Dagher, Pierre ; Balsam, Leah ; Weber, J. Todd ; Egnor, Richard W. ; Charney, Alan N. / Modulation of chloride secretion in the rat colon by intracellular bicarbonate. In: Gastroenterology. 1992 ; Vol. 103, No. 1. pp. 120-127.
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