Modulation of gene expression in transgenic mouse hearts overexpressing calsequestrin

Y. Ihara, Y. J. Suzuki, K. Kitta, Larry Jones, T. Ikeda

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Calsequestrin (CSQ) is the major Ca2+ binding protein of the cardiac sarcoplasmic reticulum (SR). Transgenic mice overexpressing CSQ at the age of 7 weeks exhibit concentric cardiac hypertrophy, and by 13 weeks the condition progresses to dilated cardiomyopathy. The present study used a differential display analysis to identify genes whose expressions are modulated in the CSQ-overexpressing mouse hearts to provide information on the mechanism of transition from concentric cardiac hypertrophy to failure. Cardiac ankyrin repeat protein (CARP), glutathione peroxidase (Gpx1), and genes which participate in the formation of extracellular matrix including decorin, TSC-36, Magp2, Osf2, and SPARC are upregulated in CSQ mouse hearts at 7 and 13 weeks of age compared to those of non-transgenic littermates. In addition, two novel genes without sequence similarities to any known genes are upregulated in CSQ-overexpressing mouse hearts. Several genes are downregulated at 13 weeks, including SR Ca2+-ATPase (SERCA2) and adenine nucleotide translocase 1 (Ant1) genes. Further, a functionally yet unknown gene (NM6586) previously identified in the mouse wolffian duct is dramatically downregulated in CSQ mice with dilated hearts. Thus, CARP, Gpx1, and genes encoding extracellular matrix proteins may participate in the development of cardiac hypertrophy and fibrosis, and changes in SERCA2, Ant1, and NM6586 mRNA expression may be involved in transition from concentric to dilated cardiac hypertrophy.

Original languageEnglish
Pages (from-to)21-29
Number of pages9
JournalCell Calcium
Volume32
Issue number1
DOIs
StatePublished - Jul 2002

Fingerprint

Calsequestrin
Transgenic Mice
Cardiomegaly
Gene Expression
ATP Translocases Mitochondrial ADP
Ankyrin Repeat
Genes
Sarcoplasmic Reticulum
Down-Regulation
Wolffian Ducts
Decorin
Calcium-Transporting ATPases
Extracellular Matrix Proteins
Dilated Cardiomyopathy
Glutathione Peroxidase
Extracellular Matrix
Carrier Proteins
Proteins
Fibrosis
Messenger RNA

ASJC Scopus subject areas

  • Cell Biology
  • Endocrinology

Cite this

Modulation of gene expression in transgenic mouse hearts overexpressing calsequestrin. / Ihara, Y.; Suzuki, Y. J.; Kitta, K.; Jones, Larry; Ikeda, T.

In: Cell Calcium, Vol. 32, No. 1, 07.2002, p. 21-29.

Research output: Contribution to journalArticle

Ihara, Y. ; Suzuki, Y. J. ; Kitta, K. ; Jones, Larry ; Ikeda, T. / Modulation of gene expression in transgenic mouse hearts overexpressing calsequestrin. In: Cell Calcium. 2002 ; Vol. 32, No. 1. pp. 21-29.
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