Modulation of interleukin-1 receptor expression and interleukin-1 response in fibroblasts by platelet-derived growth factor

P. D. Bonin, J. P. Singh

Research output: Contribution to journalArticle

42 Scopus citations

Abstract

Our studies have revealed that the interleukin-1 (IL-1) receptors and response in BALB/c/3T3 fibroblasts are modulated by platelet-derived growth factor (PDGF). Incubation of quiescent cultures of BALB/c/3T3 fibroblasts with PDGF resulted in 4-5-fold higher 125I-IL-1 binding than the untreated cultures. Scatchard analysis showed that the increased 125I-IL-1 binding by PDGF-treated cells was due to a net increase in cell surface IL-1 receptors with no apparent change in binding affinity. The PDGF-induced increase of 125I-IL-1 binding was blocked by inhibitors of transcription, suggesting that a transcriptional event, perhaps a de novo synthesis of IL-1 receptors, is required for PDGF response. In a culture medium depleted of serum factors, IL-1 alone produced only a marginal (40-50%) increase in DNA synthesis in BALB/c/3T3 cells. In cells first exposed to PDGF, IL-1 produced 8-10-fold higher DNA synthesis than the controls. These findings provide evidence that IL-1 action in fibroblast may be regulated at the level of receptor expression.

Original languageEnglish (US)
Pages (from-to)11052-11055
Number of pages4
JournalJournal of Biological Chemistry
Volume263
Issue number23
StatePublished - Jan 1 1988

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ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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