Modulation of SV-40 transformed human cornea! epithelial cell proliferation by Norepinephrine

C. J. Murphy, S. Campbell, Carl Marfurt

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Purpose: To determine the ability of norepinephrine to modulate proliferation of SV-40 transformed human corneal epithelial cells. Methods: Assays were performed using SV-40 human corneal epithelial cells (Araki-Sasaki et al. 1995. IOVS 36:614-621). For proliferation assays: cells were plated in 96 well plates coated with fibronectin and collagen. A dose response curve was generated for norepinephrine in concentrations from lOpM to lOuM. Cell number in each well was evaluated using the fluorochrome Calcein AM (Molecular Probes(r)-an intracellular esterase cleavage substrate) and fluorescence determined using an automated fluorescent plate reader. Results: Norepinephrine significantly increased cell proliferation in concentrations from lOOnM to 10 uM. Conclusions: Norepinephrine is normally found in the cornea and may play significant physiologic functions as regards maintenance of a healthy anterior corneal epithelium.

Original languageEnglish (US)
JournalInvestigative Ophthalmology and Visual Science
Volume38
Issue number4
StatePublished - 1997
Externally publishedYes

Fingerprint

Cornea
Norepinephrine
Epithelial Cells
Cell Proliferation
Molecular Probes
Corneal Epithelium
Esterases
Fluorescent Dyes
Fibronectins
Collagen
Cell Count
Fluorescence
Maintenance

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Modulation of SV-40 transformed human cornea! epithelial cell proliferation by Norepinephrine. / Murphy, C. J.; Campbell, S.; Marfurt, Carl.

In: Investigative Ophthalmology and Visual Science, Vol. 38, No. 4, 1997.

Research output: Contribution to journalArticle

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