Modulation of the platelet‐derived growth factor induced replicative response

C. D. Scher, A. P. Whipple, J. P. Singh, W. J. Pledger

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Quiescent cultures of density arrested BALB/c‐3T3 cells have been sensitized to the growth stimulatory action of the platelet‐derived growth factor (PDGF). Sensitization was achieved by depriving the cultures of PDGF prior to growth stimulation and was noted after transfer of cultures from medium supplemented with 10% serum to medium containing either an equivalent concentration of platelet‐poor plasma or a low concentration (0.5%) of serum. Sensitized cultures required less pure PDGF for growth stimulation than non‐sensitized ones. In addition such cultures required less mitogen to synthesize a PDGF modulated major excreted protein (MEP). The mechanism of sensitization was investigated. Sensitized cultures did not bind more PDGF than non‐sensitized ones. Rather, sensitization appeared to result from the loss of cells that occurred when cultures were deprived of PDGF. Such a loss increased the amount of PDGF available per cell, causing a higher percentage of cells to enter the S phase. Similarly, the amount of PDGF per cell regulated MEP synthesis. Furthermore, in non‐sensitized cultures (containing the same number of cells), the absolute quantity rather than the concentration of PDGF regulated DNA synthesis. It appears that the amount of PDGF per cell modulates mitogenesis.

Original languageEnglish (US)
Pages (from-to)10-16
Number of pages7
JournalJournal of Cellular Physiology
Volume123
Issue number1
DOIs
StatePublished - 1985
Externally publishedYes

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Intercellular Signaling Peptides and Proteins
Modulation
Growth
Serum
S Phase
Mitogens
Culture Media
Proteins
Cell Count
Plasmas
DNA

ASJC Scopus subject areas

  • Medicine(all)
  • Physiology
  • Clinical Biochemistry
  • Cell Biology

Cite this

Modulation of the platelet‐derived growth factor induced replicative response. / Scher, C. D.; Whipple, A. P.; Singh, J. P.; Pledger, W. J.

In: Journal of Cellular Physiology, Vol. 123, No. 1, 1985, p. 10-16.

Research output: Contribution to journalArticle

Scher, C. D. ; Whipple, A. P. ; Singh, J. P. ; Pledger, W. J. / Modulation of the platelet‐derived growth factor induced replicative response. In: Journal of Cellular Physiology. 1985 ; Vol. 123, No. 1. pp. 10-16.
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