Modulation of Xenopus oocyte-expressed phospholemman-induced ion currents by co-expression of protein kinases

J. Paul Mounsey, Kun Ping Lu, Manoj K. Patel, Zhenhui Chen, L. Tyler Horne, J. Edward John, Anthony R. Means, Larry Jones, J. Randall Moorman

Research output: Contribution to journalArticle

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Abstract

Phospholemman (PLM), the major sarcolemmal substrate for phosphorylation by cAMP-dependent kinase (PKA) protein kinase C (PKC) and NIMA kinase in muscle, induces hyperpolarization-activated anion currents in Xenopus oocytes, most probably by enhancing endogenous oocyte currents. PLM peptides from the cytoplasmic tail are phosphorylated by PKA at S68, by NIMA kinase at S63, and by PKC at both S63 and S68. We have confirmed the phosphorylation sites in the intact protein, and we have investigated the role of phosphorylation in the regulatory activity of PLM using oocyte expression experiments. We found: (1) the cytoplasmic domain is not essential for inducing currents in oocytes; (2) co-expression of PKA increased the amplitude of oocyte currents and the amount of PLM in the oocyte membrane largely, but not exclusively, through phosphorylation of S68; (3) co- expression of PKA had no effect on a PLM mutant in which all putative phosphorylation sites had been inactivated by serine to alanine mutation (SSST 62, 63, 68, 69 AAAA); (4) co-expression of PKC had no effect in this system; (5) co-expression of NIMA kinase increased current amplitude and membrane protein level, but did not require PLM phosphorylation. These findings point to a role for phosphorylation in the function of PLM.

Original languageEnglish
Pages (from-to)305-318
Number of pages14
JournalBiochimica et Biophysica Acta - Molecular Cell Research
Volume1451
Issue number2-3
DOIs
StatePublished - Sep 21 1999

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Xenopus
Protein Kinases
Oocytes
Phosphorylation
Ions
Phosphotransferases
Protein Kinase C
phospholemman
Alanine
Serine
Anions
Tail
Membrane Proteins
Muscles
Peptides
Mutation
Membranes
Proteins

Keywords

  • Ion channel regulation
  • Phosphorylation
  • Protein kinase

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology
  • Biophysics

Cite this

Modulation of Xenopus oocyte-expressed phospholemman-induced ion currents by co-expression of protein kinases. / Mounsey, J. Paul; Lu, Kun Ping; Patel, Manoj K.; Chen, Zhenhui; Horne, L. Tyler; John, J. Edward; Means, Anthony R.; Jones, Larry; Moorman, J. Randall.

In: Biochimica et Biophysica Acta - Molecular Cell Research, Vol. 1451, No. 2-3, 21.09.1999, p. 305-318.

Research output: Contribution to journalArticle

Mounsey, J. Paul ; Lu, Kun Ping ; Patel, Manoj K. ; Chen, Zhenhui ; Horne, L. Tyler ; John, J. Edward ; Means, Anthony R. ; Jones, Larry ; Moorman, J. Randall. / Modulation of Xenopus oocyte-expressed phospholemman-induced ion currents by co-expression of protein kinases. In: Biochimica et Biophysica Acta - Molecular Cell Research. 1999 ; Vol. 1451, No. 2-3. pp. 305-318.
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