Molecular characterization of a mammalian smooth muscle myosin light chain kinase

Patricia Gallagher, B. Herring, Suzy A. Griffin, James T. Stull

Research output: Contribution to journalArticle

110 Citations (Scopus)

Abstract

A 5.6-kilobase cDNA clone has been isolated which includes the entire coding region for the myosin light chain kinase from rabbit uterine tissue. This cDNA, expressed in COS cells, encodes a Ca2+/calmodulin-dependent protein kinase with catalytic properties similar to other purified smooth muscle myosin light chain kinases. A module (TLKPVGNIKPAE), repeated sequentially 15 times, has been identified near the N terminus of this smooth muscle kinase. It is not present in chicken gizzard or rabbit skeletal muscle myosin light chain kinases. This repeat module and a subrepeat (K P A/V) are similar in amino acid content to repeated motifs present in other proteins, some of which have been shown to associate with chromatin structures. Immunoblot analysis after sodium dodecyl sulfate-polyacrylamide gel electrophoresis, used to compare myosin light chain kinase present in rabbit, bovine, and chicken smooth and nonmuscle tissues, showed that within each species both tissue types have myosin light chain kinases with indistinguishable molecular masses. These data suggest that myosin light chain kinases present in smooth and nonmuscle tissues are the same protein.

Original languageEnglish (US)
Pages (from-to)23936-23944
Number of pages9
JournalJournal of Biological Chemistry
Volume266
Issue number35
StatePublished - Dec 15 1991
Externally publishedYes

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Smooth Muscle Myosins
Myosin-Light-Chain Kinase
Tissue
Rabbits
Chickens
Complementary DNA
Skeletal Muscle Myosins
Avian Gizzard
Calcium-Calmodulin-Dependent Protein Kinases
COS Cells
Molecular mass
Electrophoresis
Sodium Dodecyl Sulfate
Chromatin
Smooth Muscle
Muscle
Polyacrylamide Gel Electrophoresis
Proteins
Phosphotransferases
Clone Cells

ASJC Scopus subject areas

  • Biochemistry

Cite this

Gallagher, P., Herring, B., Griffin, S. A., & Stull, J. T. (1991). Molecular characterization of a mammalian smooth muscle myosin light chain kinase. Journal of Biological Chemistry, 266(35), 23936-23944.

Molecular characterization of a mammalian smooth muscle myosin light chain kinase. / Gallagher, Patricia; Herring, B.; Griffin, Suzy A.; Stull, James T.

In: Journal of Biological Chemistry, Vol. 266, No. 35, 15.12.1991, p. 23936-23944.

Research output: Contribution to journalArticle

Gallagher, P, Herring, B, Griffin, SA & Stull, JT 1991, 'Molecular characterization of a mammalian smooth muscle myosin light chain kinase', Journal of Biological Chemistry, vol. 266, no. 35, pp. 23936-23944.
Gallagher P, Herring B, Griffin SA, Stull JT. Molecular characterization of a mammalian smooth muscle myosin light chain kinase. Journal of Biological Chemistry. 1991 Dec 15;266(35):23936-23944.
Gallagher, Patricia ; Herring, B. ; Griffin, Suzy A. ; Stull, James T. / Molecular characterization of a mammalian smooth muscle myosin light chain kinase. In: Journal of Biological Chemistry. 1991 ; Vol. 266, No. 35. pp. 23936-23944.
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