Molecular characterization of a novel cDNA from murine cerebellum, developmental expression, and distribution in brain

M. Kambouris, L. Sangameswaran, L. C. Triarhou, C. A. Kozak, Stephen Dlouhy, Bernardino Ghetti, M. E. Hodes

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Several novel cDNA clones were previously identified by immunoscreening a cerebellar cDNA expression library derived from heterozygous weaver (wv/ +) mice at postnatal day one (P1) with an antigranule cell antiserum. One cDNA, GCAP-8 (granule cell antiserum-positive clone 8) has been further characterized. The 1.1 kb insert is a partial cDNA containing a segment near the 3′ end of the full-length cDNA. The 5′ end of the GCAP-8 cDNA contains a 259 nucleotide open reading frame (ORF) coding for the last 85 amino acids of the carboxy terminus of the encoded protein. The encoded polypeptide contains two highly hydrophobic segments interrupted by a basic stretch. The carboxy terminus of this protein is cysteine-rich, with 10 cysteine residues among the 85 amino acids. The GCAP-8 cDNA probably represents a single-copy gene. The GCAP-8 gene, designated Gcap1, was mapped to the distal region of mouse chromosome 5 by the analyses of two multilocus crosses. The distribution of the GCAP-8 mRNA in mouse brain was studied by in situ hybridization histochemistry. In the adult mouse brain, strong hybridization was detected in cerebellum, hippocampus, substantia nigra (SN), and cerebral cortex. In mouse cerebellum, hybridization was detected in granule cells, Purkinje cells, and in cells of the deep cerebellar nuclei (DCN). In human cerebellum, hybridization was detected in the granule cell layer. In the mouse, GCAP-8 is expressed at least as early as embryonic day 14 (E14) in the central nervous system (CNS).

Original languageEnglish
Pages (from-to)192-199
Number of pages8
JournalMolecular Brain Research
Volume25
Issue number3-4
DOIs
StatePublished - 1994

Fingerprint

Cerebellum
Complementary DNA
Immune Sera
Brain
Clone Cells
Cysteine
Neurologic Mutant Mice
Amino Acids
Cerebellar Nuclei
Chromosomes, Human, Pair 5
Purkinje Cells
Substantia Nigra
Gene Library
Cerebral Cortex
Open Reading Frames
Genes
In Situ Hybridization
Hippocampus
Proteins
Nucleotides

Keywords

  • cDNA
  • Cerebellum
  • Granule cell
  • Hippocampal formation
  • Human
  • In situ hybridization
  • Mouse
  • Olfactory bulb
  • Substantia nigra

ASJC Scopus subject areas

  • Molecular Biology
  • Cellular and Molecular Neuroscience

Cite this

Molecular characterization of a novel cDNA from murine cerebellum, developmental expression, and distribution in brain. / Kambouris, M.; Sangameswaran, L.; Triarhou, L. C.; Kozak, C. A.; Dlouhy, Stephen; Ghetti, Bernardino; Hodes, M. E.

In: Molecular Brain Research, Vol. 25, No. 3-4, 1994, p. 192-199.

Research output: Contribution to journalArticle

Kambouris, M. ; Sangameswaran, L. ; Triarhou, L. C. ; Kozak, C. A. ; Dlouhy, Stephen ; Ghetti, Bernardino ; Hodes, M. E. / Molecular characterization of a novel cDNA from murine cerebellum, developmental expression, and distribution in brain. In: Molecular Brain Research. 1994 ; Vol. 25, No. 3-4. pp. 192-199.
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