Molecular characterization of testicular germ cell tumors using tissue microdissection

Liang Cheng, Steven A. Mann, Antonio Lopez-Beltran, Michal Chovanec, Matteo Santoni, Mingsheng Wang, Costantine Albany, Nabil Adra, Darrell D. Davidson, Alessia Cimadamore, Rodolfo Montironi, Shaobo Zhang

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Testicular germ cell tumors are among the most common malignancies seen in children and young adults. Genomic studies have identified characteristic molecular profiles in testicular cancer, which are associated with histologic subtypes and may predict clinical behavior including treatment responses. Emerging molecular technologies analyzing tumor genomics, transcriptomics, and proteomics may now guide precision management of testicular tumors. Laser-assisted microdissection methods such as laser capture microdissection efficiently isolate selected tumor cells from routine pathology specimens, avoiding contamination from nontarget cell populations. Laser capture microdissection in combination with next generation sequencing makes precise high throughput genetic evaluation effective and efficient. The use of laser capture microdissection (LCM) for molecular testing may translate into great benefits for the clinical management of patients with testicular cancers. This review discusses application protocols for laser-assisted microdissection to investigate testicular germ cell tumors.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages31-47
Number of pages17
DOIs
StatePublished - 2021

Publication series

NameMethods in Molecular Biology
Volume2195
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Germ cell tumors
  • Laser capture microdissection
  • Molecular genetics
  • Testicular cancer
  • Tissue microdissection

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Fingerprint Dive into the research topics of 'Molecular characterization of testicular germ cell tumors using tissue microdissection'. Together they form a unique fingerprint.

  • Cite this

    Cheng, L., Mann, S. A., Lopez-Beltran, A., Chovanec, M., Santoni, M., Wang, M., Albany, C., Adra, N., Davidson, D. D., Cimadamore, A., Montironi, R., & Zhang, S. (2021). Molecular characterization of testicular germ cell tumors using tissue microdissection. In Methods in Molecular Biology (pp. 31-47). (Methods in Molecular Biology; Vol. 2195). Humana Press Inc.. https://doi.org/10.1007/978-1-0716-0860-9_3