Molecular characterization of the creatine kinases and some historical perspectives

Wenning Qin, Zaza Khuchua, Judy Cheng, Jaime Boero, R. Payne, Arnold W. Strauss

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

Over the last 15 years, molecular characterization of the creatine kinase (CK) gene family has paralleled the molecular revolution of understanding gene structure, function, and regulation. In this review, we present a summary of advances in molecular analysis of the CK gene family with a few vignettes of historical interest. We describe how the muscle CK gene provided an essential model system to examine myogenic regulatory mechanisms, leading to the discovery of the binding site for the MyoD family of basic helix-loop-helix transcription factors essential in skeletal myogenesis and the characterization of the MEF2 family of factors with an A/T rich consensus binding site essential in skeletal myogenesis and cardiogenesis. Cloning and characterization of the four mRNAs and nuclear genes encoding the cytosolic CKs, muscle and brain CKs, and the mitochondrial (Mt) CKs, sarcomeric MtCK and ubiquitous MtCK, has allowed intriguing study of tissue-specific and cell-specific expression of the different CKs and analysis of structural, functional, regulatory, and evolutionary relationships among both the four CK proteins and genes. Current and future studies focus on understanding both cellular energetics facilitated by the CK enzymes, especially energy channelling from the site of production, the mitochondrial matrix and inner membrane, to various cytosolic foci of utilization, and regulation of MtCK gene expression at the cell and tissue-specific level as models of regulation of energy producing genes.

Original languageEnglish (US)
Pages (from-to)153-167
Number of pages15
JournalMolecular and Cellular Biochemistry
Volume184
Issue number1-2
StatePublished - 1998
Externally publishedYes

Fingerprint

Creatine Kinase
Genes
Muscle Development
Binding Sites
MM Form Creatine Kinase
Tissue
Basic Helix-Loop-Helix Transcription Factors
Gene encoding
Cloning
Essential Genes
Gene Expression Regulation
Mitochondrial Membranes
Gene expression
Muscle
Organism Cloning
Brain
Membranes
Muscles
Messenger RNA
Enzymes

Keywords

  • Creatine kinase
  • Energy
  • Gene family
  • Mitochondrion
  • Myogenesis
  • Transcription factors

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Qin, W., Khuchua, Z., Cheng, J., Boero, J., Payne, R., & Strauss, A. W. (1998). Molecular characterization of the creatine kinases and some historical perspectives. Molecular and Cellular Biochemistry, 184(1-2), 153-167.

Molecular characterization of the creatine kinases and some historical perspectives. / Qin, Wenning; Khuchua, Zaza; Cheng, Judy; Boero, Jaime; Payne, R.; Strauss, Arnold W.

In: Molecular and Cellular Biochemistry, Vol. 184, No. 1-2, 1998, p. 153-167.

Research output: Contribution to journalArticle

Qin, W, Khuchua, Z, Cheng, J, Boero, J, Payne, R & Strauss, AW 1998, 'Molecular characterization of the creatine kinases and some historical perspectives', Molecular and Cellular Biochemistry, vol. 184, no. 1-2, pp. 153-167.
Qin, Wenning ; Khuchua, Zaza ; Cheng, Judy ; Boero, Jaime ; Payne, R. ; Strauss, Arnold W. / Molecular characterization of the creatine kinases and some historical perspectives. In: Molecular and Cellular Biochemistry. 1998 ; Vol. 184, No. 1-2. pp. 153-167.
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