Molecular cloning and characterization of additional factors that bind to the interferon-α/β response element B of the murine (2′-5′)oligoadenylate synthetase ME-12 gene

Cong Yan, Igor Tamm

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The function of interferon (IFN) response elements (IREs) may be classified into two classes according to the location of the IRE in the polymerase II transcription promoter: located upstream of the core promoter, or located within the core promoter. Thus, the IRE-binding factors (IREBFs) can serve as sequence-specific transcription factors that activate IFN-inducible gene transcription and they can participate in preinitiation complex formation. In the murine and human (2′-5′)oligoadenylate synthetase genes the IRE is located within the core promoter region. For this reason, it is important to isolate cDNAs encoding protein factors that can bind to this IRE and determine their functional roles in the regulation of expression of the IFN-inducible (2′-5′)oligoadenylate synthetase gene. We have isolated three groups of cDNAs that encode IREBFs. Here we report on clone 38 cDNA, which contains an open reading frame encoding a protein of 277 amino acids that is designated IREBF-2. The C terminus of IREBF-2 is extremely proline-rich and contains a high percentage of short-armed amino acids. IREBF-2 shares marked similarity with some gene products of the herpes group of viruses. These gene products are potent transcription activators. IREBF-2 is constitutively expressed in BALB/c 3T3 cells.

Original languageEnglish (US)
Pages (from-to)8859-8863
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume89
Issue number19
StatePublished - Oct 1 1992
Externally publishedYes

Fingerprint

Molecular Cloning
Response Elements
Ligases
Interferons
Complementary DNA
Genes
BALB 3T3 Cells
Amino Acids
Genetic Promoter Regions
Proline
Open Reading Frames
Proteins
Transcription Factors
Clone Cells
2',5'-oligoadenylate
Viruses

Keywords

  • Gene regulation
  • Interferon signal transduction pathways
  • Proline-rich domain
  • Transcription factors

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

@article{a3f750d4fec94068a0c65f1b037db734,
title = "Molecular cloning and characterization of additional factors that bind to the interferon-α/β response element B of the murine (2′-5′)oligoadenylate synthetase ME-12 gene",
abstract = "The function of interferon (IFN) response elements (IREs) may be classified into two classes according to the location of the IRE in the polymerase II transcription promoter: located upstream of the core promoter, or located within the core promoter. Thus, the IRE-binding factors (IREBFs) can serve as sequence-specific transcription factors that activate IFN-inducible gene transcription and they can participate in preinitiation complex formation. In the murine and human (2′-5′)oligoadenylate synthetase genes the IRE is located within the core promoter region. For this reason, it is important to isolate cDNAs encoding protein factors that can bind to this IRE and determine their functional roles in the regulation of expression of the IFN-inducible (2′-5′)oligoadenylate synthetase gene. We have isolated three groups of cDNAs that encode IREBFs. Here we report on clone 38 cDNA, which contains an open reading frame encoding a protein of 277 amino acids that is designated IREBF-2. The C terminus of IREBF-2 is extremely proline-rich and contains a high percentage of short-armed amino acids. IREBF-2 shares marked similarity with some gene products of the herpes group of viruses. These gene products are potent transcription activators. IREBF-2 is constitutively expressed in BALB/c 3T3 cells.",
keywords = "Gene regulation, Interferon signal transduction pathways, Proline-rich domain, Transcription factors",
author = "Cong Yan and Igor Tamm",
year = "1992",
month = "10",
day = "1",
language = "English (US)",
volume = "89",
pages = "8859--8863",
journal = "Proceedings of the National Academy of Sciences of the United States of America",
issn = "0027-8424",
number = "19",

}

TY - JOUR

T1 - Molecular cloning and characterization of additional factors that bind to the interferon-α/β response element B of the murine (2′-5′)oligoadenylate synthetase ME-12 gene

AU - Yan, Cong

AU - Tamm, Igor

PY - 1992/10/1

Y1 - 1992/10/1

N2 - The function of interferon (IFN) response elements (IREs) may be classified into two classes according to the location of the IRE in the polymerase II transcription promoter: located upstream of the core promoter, or located within the core promoter. Thus, the IRE-binding factors (IREBFs) can serve as sequence-specific transcription factors that activate IFN-inducible gene transcription and they can participate in preinitiation complex formation. In the murine and human (2′-5′)oligoadenylate synthetase genes the IRE is located within the core promoter region. For this reason, it is important to isolate cDNAs encoding protein factors that can bind to this IRE and determine their functional roles in the regulation of expression of the IFN-inducible (2′-5′)oligoadenylate synthetase gene. We have isolated three groups of cDNAs that encode IREBFs. Here we report on clone 38 cDNA, which contains an open reading frame encoding a protein of 277 amino acids that is designated IREBF-2. The C terminus of IREBF-2 is extremely proline-rich and contains a high percentage of short-armed amino acids. IREBF-2 shares marked similarity with some gene products of the herpes group of viruses. These gene products are potent transcription activators. IREBF-2 is constitutively expressed in BALB/c 3T3 cells.

AB - The function of interferon (IFN) response elements (IREs) may be classified into two classes according to the location of the IRE in the polymerase II transcription promoter: located upstream of the core promoter, or located within the core promoter. Thus, the IRE-binding factors (IREBFs) can serve as sequence-specific transcription factors that activate IFN-inducible gene transcription and they can participate in preinitiation complex formation. In the murine and human (2′-5′)oligoadenylate synthetase genes the IRE is located within the core promoter region. For this reason, it is important to isolate cDNAs encoding protein factors that can bind to this IRE and determine their functional roles in the regulation of expression of the IFN-inducible (2′-5′)oligoadenylate synthetase gene. We have isolated three groups of cDNAs that encode IREBFs. Here we report on clone 38 cDNA, which contains an open reading frame encoding a protein of 277 amino acids that is designated IREBF-2. The C terminus of IREBF-2 is extremely proline-rich and contains a high percentage of short-armed amino acids. IREBF-2 shares marked similarity with some gene products of the herpes group of viruses. These gene products are potent transcription activators. IREBF-2 is constitutively expressed in BALB/c 3T3 cells.

KW - Gene regulation

KW - Interferon signal transduction pathways

KW - Proline-rich domain

KW - Transcription factors

UR - http://www.scopus.com/inward/record.url?scp=0026767468&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026767468&partnerID=8YFLogxK

M3 - Article

C2 - 1409578

AN - SCOPUS:0026767468

VL - 89

SP - 8859

EP - 8863

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

SN - 0027-8424

IS - 19

ER -