Molecular cloning and characterization of an SRCAP chromatin remodeling homologue in Toxoplasma gondii

William J. Sullivan, M. Alexandra Monroy, Wolfgang Bohne, Karuna C. Nallani, John Chrivia, Peter Yaciuk, Charles K. Smith, Sherry F. Queener

Research output: Contribution to journalArticle

21 Scopus citations

Abstract

We have identified and mapped a gene in Toxoplasma gondii that encodes a homologue of SRCAP (Snf2-related CBP activator protein), a member of the SNF/SWI family of chromatin remodeling factors. The genomic locus (TgSRCAP) is present as a single copy and contains 16 introns. The predicted cDNA contains an open reading frame of 8,775 bp and encodes a protein of 2,924 amino acids. We have identified additional SRCAP-like sequences in Apicomplexa for comparison by screening genomic databases. An analysis of SRCAP homologues between species reveals signature features that may be indicative of SRCAP members. Expression of mRNA encoding TgSRCAP is upregulated when tachyzoite (invasive form) parasites are induced to differentiate into bradyzoites (encysted form) in vitro. Recombinant TgSRCAP protein is functionally equivalent to the human homologue, being capable of increasing transcription mediated by CREB.

Original languageEnglish (US)
Pages (from-to)1-8
Number of pages8
JournalParasitology Research
Volume90
Issue number1
StatePublished - May 1 2003

ASJC Scopus subject areas

  • Parasitology
  • veterinary(all)
  • Insect Science
  • Infectious Diseases

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    Sullivan, W. J., Monroy, M. A., Bohne, W., Nallani, K. C., Chrivia, J., Yaciuk, P., Smith, C. K., & Queener, S. F. (2003). Molecular cloning and characterization of an SRCAP chromatin remodeling homologue in Toxoplasma gondii. Parasitology Research, 90(1), 1-8.