Molecular cloning and characterization of interferon α/β response element binding factors of the murine (2′-5′)oligoadenylate synthetase ME-12 gene

Cong Yan, Igor Tamm

Research output: Contribution to journalArticlepeer-review

39 Scopus citations

Abstract

Seven clones encoding interferon response element binding factors have been isolated from a mouse fibroblast λgt11 cDNA library by using a 32P end-labeled tandem trimer of the mouse (2′-5′)oligoadenylate synthetase gene Interferon response element as a probe. Clone 16 shares strong similarity (95%) at both DNA and amino acid level with YB-1, a human major histocompatibility complex class II Y-box DNA-binding protein, and with dbpB, a human epidermal growth factor receptor gene enhancer region binding protein. The product of the gene represented by clone 16 may represent a factor that regulates multiple genes by binding to a variety of 5′ regulatory elements. Clone 25 is a 2407-base-pair-long cDNA and contains a putative 311-amino acid open reading frame corresponding to an estimated mass of 35.5 kDa. This putative protein, designated as interferon response element binding factor 1 (IREBF-1), contains an acidic domain, three heptad repeat leucine arrays, and a region that shares similarity with the yeast transcriptional factor GAL4 DNA-binding domain. Furthermore, the C terminus of IREBF-1 shows an unusual amphipathic property: within a 79-amino acid range, one side of the α-helical region contains a preponderance of hydrophobic amino acids and the other side contains hydrophilic amino acids. This type of structure provides a strong hydrophobic force for protein-protein interaction.

Original languageEnglish (US)
Pages (from-to)144-148
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume88
Issue number1
DOIs
StatePublished - Jan 1 1991

Keywords

  • (2′-5′)oligoadenylate synthetase gene regulation
  • Amphipathic protein
  • Interferon signal transduction pathways
  • Transcription factors

ASJC Scopus subject areas

  • General

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