Molecular cloning and characterization of SPAP1, an inhibitory receptor

M. J. Xu, R. Zhao, Z. J. Zhao

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

We have cloned a novel cell-surface protein designated SPAP1a for SH2 domain-containing phosphatase anchor protein 1a. SPAP1a belongs to the group of type I transmembrane proteins. Its extracellular domain contains a single immunoglobulin-like domain, and its intracellular segment has two immunoreceptor tyrosine-based inhibition motifs (ITIMs). We also identified two alternatively spliced products that were named SPAP1b and SPAP1c. SPAP1b contains a short intracellular part without ITIMs, while SPAP1c lacks the transmembrane segment and represents a potential soluble protein. Sequence alignment with the genomic database revealed that the SPAP1 gene contains seven exons and is localized at chromosome 1q21. PCR analyses demonstrated that SPAP1a mRNA is specifically expressed in human hematopoietic tissues including spleen, peripheral blood, and bone marrow, and it may be restricted to expression in B cells. Recombinant SPAP1a is tyrosine phosphorylated in cells upon pervanadate stimulation and tyrosine-phosphorylated SPAP1a recruits the SH2 domain containing phosphatase SHP-1, but not SHP-2. As a specific anchor protein of SHP-1, SPAP1a may have an important role in hematopoietic cell signaling.

Original languageEnglish (US)
Pages (from-to)768-775
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume280
Issue number3
DOIs
StatePublished - 2001
Externally publishedYes

Fingerprint

Immunoreceptor Tyrosine-Based Inhibition Motif
Cloning
Molecular Cloning
Tyrosine
src Homology Domains
Non-Receptor Type 6 Protein Tyrosine Phosphatase
Anchors
Proteins
Sequence Alignment
Phosphoprotein Phosphatases
Cell signaling
Exons
Membrane Proteins
B-Lymphocytes
Spleen
Chromosomes
Bone Marrow
Phosphoric Monoester Hydrolases
Databases
Immunoglobulins

Keywords

  • Characterization
  • Cloning
  • Ig family
  • ITIM
  • Tyrosine phosphatase

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Molecular cloning and characterization of SPAP1, an inhibitory receptor. / Xu, M. J.; Zhao, R.; Zhao, Z. J.

In: Biochemical and Biophysical Research Communications, Vol. 280, No. 3, 2001, p. 768-775.

Research output: Contribution to journalArticle

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