Molecular cloning and regulation of expression of the genes for initiation factor 3 and two aminoacyl-tRNA synthetases

D. Elseviers, P. Gallagher, A. Hoffman, B. Weinberg, I. Schwartz

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

A 22-kilobase fragment of the E. coli chromosome which contains the genes for translation initiation factor 3, phenylalanyl-tRNA synthetase, and threonyl-tRNA synthetase was cloned into plasmid pACYC184. The hybrid plasmid (designated pID1) complements a temperature-sensitive pheS lesion in E. coli np37. pID1-transformed NP37 overproduce initiation factor 3 and phenylalanyl-tRNA synthetase. Gene expression from pID1 was studied in vitro in a coupled transcription-translation system and in minicells. The results suggest that the genes for initiation factor 3 and phenylalanyl- and threonyl-tRNA synthetase are regulated by different mechanisms.

Original languageEnglish (US)
Pages (from-to)357-362
Number of pages6
JournalJournal of bacteriology
Volume152
Issue number1
StatePublished - 1982

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

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    Elseviers, D., Gallagher, P., Hoffman, A., Weinberg, B., & Schwartz, I. (1982). Molecular cloning and regulation of expression of the genes for initiation factor 3 and two aminoacyl-tRNA synthetases. Journal of bacteriology, 152(1), 357-362.