Molecular cloning and sequencing of pheU, a gene for Escherichia coli tRNAPhe

Ira Schwartz, Robin Ann Klotsky, Dirk Elseviers, Patricia J. Gallagher, Manuel Krauskopf, M. A.Q. Siddiqui, James F.H. Wong, Bruce A. Roe

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

A recombinant plasmid (designated pID2) carrying the E. coli gene for tRNAPhe has been isolated from a plasmid bank constructed by the ligation of a total EcoRI digest of E. coli K12 DNA into the EcoRI site of pACYC184 DNA. The plasmid was selected by virtue of its ability to complement a temperature-sensitive lesion in the gene (PheS) for the α-subunit of phenylalanyl-tRNA synthetase. Crude tRNA isolated from such transformants exhibited elevated levels of phenylalanine acceptor activity. The tRNAPhe gene has been localized within the first 300 base pairs of a 3.6 kb SalI fragment of pID2. The sequence of the gene and its flanking regions is presented.

Original languageEnglish (US)
Pages (from-to)4379-4389
Number of pages11
JournalNucleic acids research
Volume11
Issue number13
DOIs
StatePublished - Jul 11 1983

Fingerprint

RNA, Transfer, Phe
Cloning
Molecular Cloning
Escherichia coli
Escherichia Coli
Sequencing
Genes
Gene
Plasmids
DNA
Phenylalanine-tRNA Ligase
Escherichia coli K12
Transfer RNA
Phenylalanine
Base Pairing
Ligation
Fragment
Complement
Temperature

ASJC Scopus subject areas

  • Genetics

Cite this

Schwartz, I., Klotsky, R. A., Elseviers, D., Gallagher, P. J., Krauskopf, M., Siddiqui, M. A. Q., ... Roe, B. A. (1983). Molecular cloning and sequencing of pheU, a gene for Escherichia coli tRNAPhe. Nucleic acids research, 11(13), 4379-4389. https://doi.org/10.1093/nar/11.13.4379

Molecular cloning and sequencing of pheU, a gene for Escherichia coli tRNAPhe. / Schwartz, Ira; Klotsky, Robin Ann; Elseviers, Dirk; Gallagher, Patricia J.; Krauskopf, Manuel; Siddiqui, M. A.Q.; Wong, James F.H.; Roe, Bruce A.

In: Nucleic acids research, Vol. 11, No. 13, 11.07.1983, p. 4379-4389.

Research output: Contribution to journalArticle

Schwartz, I, Klotsky, RA, Elseviers, D, Gallagher, PJ, Krauskopf, M, Siddiqui, MAQ, Wong, JFH & Roe, BA 1983, 'Molecular cloning and sequencing of pheU, a gene for Escherichia coli tRNAPhe', Nucleic acids research, vol. 11, no. 13, pp. 4379-4389. https://doi.org/10.1093/nar/11.13.4379
Schwartz I, Klotsky RA, Elseviers D, Gallagher PJ, Krauskopf M, Siddiqui MAQ et al. Molecular cloning and sequencing of pheU, a gene for Escherichia coli tRNAPhe. Nucleic acids research. 1983 Jul 11;11(13):4379-4389. https://doi.org/10.1093/nar/11.13.4379
Schwartz, Ira ; Klotsky, Robin Ann ; Elseviers, Dirk ; Gallagher, Patricia J. ; Krauskopf, Manuel ; Siddiqui, M. A.Q. ; Wong, James F.H. ; Roe, Bruce A. / Molecular cloning and sequencing of pheU, a gene for Escherichia coli tRNAPhe. In: Nucleic acids research. 1983 ; Vol. 11, No. 13. pp. 4379-4389.
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