Molecular cloning and sequencing of pheU, a gene for Escherichia coli tRNAPhe

Ira Schwartz, Robin Ann Klotsky, Dirk Elseviers, Patricia J. Gallagher, Manuel Krauskopf, M. A.Q. Siddiqui, James F.H. Wong, Bruce A. Roe

Research output: Contribution to journalArticle

18 Scopus citations

Abstract

A recombinant plasmid (designated pID2) carrying the E. coli gene for tRNAPhe has been isolated from a plasmid bank constructed by the ligation of a total EcoRI digest of E. coli K12 DNA into the EcoRI site of pACYC184 DNA. The plasmid was selected by virtue of its ability to complement a temperature-sensitive lesion in the gene (PheS) for the α-subunit of phenylalanyl-tRNA synthetase. Crude tRNA isolated from such transformants exhibited elevated levels of phenylalanine acceptor activity. The tRNAPhe gene has been localized within the first 300 base pairs of a 3.6 kb SalI fragment of pID2. The sequence of the gene and its flanking regions is presented.

Original languageEnglish (US)
Pages (from-to)4379-4389
Number of pages11
JournalNucleic acids research
Volume11
Issue number13
DOIs
StatePublished - Jul 11 1983

ASJC Scopus subject areas

  • Genetics

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    Schwartz, I., Klotsky, R. A., Elseviers, D., Gallagher, P. J., Krauskopf, M., Siddiqui, M. A. Q., Wong, J. F. H., & Roe, B. A. (1983). Molecular cloning and sequencing of pheU, a gene for Escherichia coli tRNAPhe. Nucleic acids research, 11(13), 4379-4389. https://doi.org/10.1093/nar/11.13.4379