Molecular cloning, expression, and chromosomal localization of a human brain-specific Na +-dependent inorganic phosphate cotransporter

Binhui Ni, Yansheng Du, Xin Wu, Bradley S. DeHoff, Paul R. Rosteck, Steven M. Paul

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

We describe the molecular cloning of a cDNA encoding a human brain Na +- dependent inorganic phosphate (P(i)) cotransporter (hBNPI). The nucleotide and deduced amino acid sequences of hBNPI reveal a protein of 560 amino acids with six to eight putative transmembrane segments. hBNPI shares a high degree of homology with other Na +-dependent inorganic P(i) cotransporters, including those found in rat brain and human and rabbit kidney. Expression of hBNPI in COS-1 cells results in Na +-dependent P(i) uptake. Northern blot analysis demonstrates that hBNPI mRNA is expressed predominantly in brain and most abundantly in neuron-enriched regions such as the amygdala and hippocampus. Moderate levels of expression are also observed in glia-enriched areas such as the corpus callosum, and low levels are observed in the substantia nigra, subthalamic nuclei, and thalamus. In situ hybridization histochemistry reveals relatively high levels of hBNPI mRNA in pyramidal neurons of the cerebral cortex and hippocampus and in granule neurons of dentate gyrus. The level of hBNPI mRNA is quite low in fetal compared with adult human brain, suggesting developmental regulation of hBNPI gene expression. Southern analyses of nine eukaryotic genomic DNAs probed under stringent conditions with hBNPI cDNA revealed that the hBNPI gene is highly conserved during vertebrate evolution and that each gene is most likely present as a single copy. Using fluorescent in situ hybridization, we localized hBNPI to the long arm of chromosome 19 (19q13) in close proximity to the late-onset familial Alzheimer's disease locus.

Original languageEnglish (US)
Pages (from-to)2227-2238
Number of pages12
JournalJournal of Neurochemistry
Volume66
Issue number6
StatePublished - Jun 1996

Fingerprint

Vesicular Glutamate Transport Protein 1
Cloning
Molecular Cloning
Brain
Neurons
Messenger RNA
Developmental Gene Expression Regulation
Hippocampus
Complementary DNA
Genes
Chromosomes, Human, Pair 19
Amino Acids
Subthalamic Nucleus
Corpus Callosum
Pyramidal Cells
COS Cells
Dentate Gyrus
Substantia Nigra
Chromosomes
Amygdala

Keywords

  • Brain Na -dependent inorganic phosphate cotransporter
  • Chromosome localization
  • Molecular cloning
  • Phosphate transport and cellular metabolism

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

Molecular cloning, expression, and chromosomal localization of a human brain-specific Na +-dependent inorganic phosphate cotransporter. / Ni, Binhui; Du, Yansheng; Wu, Xin; DeHoff, Bradley S.; Rosteck, Paul R.; Paul, Steven M.

In: Journal of Neurochemistry, Vol. 66, No. 6, 06.1996, p. 2227-2238.

Research output: Contribution to journalArticle

Ni, Binhui ; Du, Yansheng ; Wu, Xin ; DeHoff, Bradley S. ; Rosteck, Paul R. ; Paul, Steven M. / Molecular cloning, expression, and chromosomal localization of a human brain-specific Na +-dependent inorganic phosphate cotransporter. In: Journal of Neurochemistry. 1996 ; Vol. 66, No. 6. pp. 2227-2238.
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