Molecular Cloning of a Novel Human cDNA on Chromosome 1q21 and Its Mouse Homolog Encoding a Nuclear Protein with DNA-Binding Ability

I. Horikawa, Hiromi Tanaka, Y. Yuasa, M. Suzuki, M. Oshimura

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

We previously reported that human chromosome 1q21 or 1q23-q24 carries a transformation suppressor gene(s) for Kirsten sarcoma virus-transformed NIH3T3 cells. In this study, we have isolated a novel human cDNA on 1q21 (designated as YL-1) as a candidate, then cloned its mouse homolog. Human and mouse YL-1 cDNA shared 96% in amino acid homology and presumably encoded a transcription factor-like polypeptide. An analysis using a specific antibody revealed the nuclear localization of YL-1 protein. The bacterially expressed YL-1 could bind to DNA. Thus, YL-1 protein possibly functions as a transcriptional regulator.

Original languageEnglish (US)
Pages (from-to)999-1007
Number of pages9
JournalBiochemical and Biophysical Research Communications
Volume208
Issue number3
DOIs
StatePublished - Mar 28 1995
Externally publishedYes

Fingerprint

Cloning
Molecular Cloning
Chromosomes
Nuclear Proteins
Kirsten murine sarcoma virus
Complementary DNA
Suppressor Genes
DNA
Human Chromosomes
Viruses
Proteins
Transcription Factors
Genes
Amino Acids
Peptides
Antibodies

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology
  • Biophysics
  • Biochemistry

Cite this

Molecular Cloning of a Novel Human cDNA on Chromosome 1q21 and Its Mouse Homolog Encoding a Nuclear Protein with DNA-Binding Ability. / Horikawa, I.; Tanaka, Hiromi; Yuasa, Y.; Suzuki, M.; Oshimura, M.

In: Biochemical and Biophysical Research Communications, Vol. 208, No. 3, 28.03.1995, p. 999-1007.

Research output: Contribution to journalArticle

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