Molecular cloning of a novel human cDNA on chromosome 1q21 and its mouse homolog encoding a nuclear protein with DNA-binding ability

Izumi Horikawa, Hiromi Tanaka, Yoshihiro Yuasa, Mikio Suzuki, Mitsuo Oshimura

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

We previously reported that human chromosome 1q21 or 1q23-q24 carries a transformation suppressor gene(s) for Kirsten sarcoma virus-transformed NIH3T3 cells. In this study, we have isolated a novel human cDNA on 1q21 (designated as YL-1) as a candidate, then cloned its mouse homolog. Human and mouse YL-1 cDNA shared 96% in amino acid homology and presumably encoded a transcription factor-like polypeptide. An analysis using a specific antibody revealed the nuclear localization of YL-1 protein. The bacterially expressed YL-1 could bind to DNA. Thus, YL-1 protein possibly functions as a transcriptional regulator.

Original languageEnglish (US)
Pages (from-to)999-1007
Number of pages9
JournalBiochemical and Biophysical Research Communications
Volume208
Issue number3
DOIs
StatePublished - Mar 28 1995

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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