Molecular Cloning of Leukotactin-1: A Novel Human β-Chemokine, a Chemoattractant for Neutrophils, Monocytes, and Lymphocytes, and a Potent Agonist at CC Chemokine Receptors 1 and 3

Byung S. Youn, Shang M. Zhang, Eun K. Lee, Doo H. Park, Hal E. Broxmeyer, Philip M. Murphy, Massimo Locati, James E. Pease, Kack K. Kim, Kathleen Antol, Byoung S. Kwon

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Abstract

A new member of human β-chemokine cDNA was isolated and named leukotactin-1 (Lkn-1). Lkn-1, along with murine macrophage inflammatory protein-related protein-1 and -2, defines a subgroup of β-chemokines based on two conserved cysteines in addition to the four others conserved in all β-chemokines. The putative mature Lkn-1 is composed of 92 amino acids with a calculated m.w. of 10,162. The Lkn-1 gene was mapped to human chromosome 17, region q12. Recombinant Lkn-1 was a potent chemoattractant for neutrophils, monocytes, and lymphocytes and induced calcium flux in these cells. Lkn-1 specifically induced calcium flux in CCR1- and CCR3-expressing HOS cell lines. Lkn-1 suppressed colony formation by human granulocyte-macrophage, erythroid, and multipotential progenitor cells stimulated by combinations of growth factors. Hence, we have isolated and characterized a human C6 β-chemokine that is a potent agonist at CCR1 and CCR3 and shows broad biologic activities, including leukocyte chemoattraction.

Original languageEnglish (US)
Pages (from-to)5201-5205
Number of pages5
JournalJournal of Immunology
Volume159
Issue number11
StatePublished - Dec 1 1997

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ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Youn, B. S., Zhang, S. M., Lee, E. K., Park, D. H., Broxmeyer, H. E., Murphy, P. M., Locati, M., Pease, J. E., Kim, K. K., Antol, K., & Kwon, B. S. (1997). Molecular Cloning of Leukotactin-1: A Novel Human β-Chemokine, a Chemoattractant for Neutrophils, Monocytes, and Lymphocytes, and a Potent Agonist at CC Chemokine Receptors 1 and 3. Journal of Immunology, 159(11), 5201-5205.