Abstract
The rat liver E1β subunit of the branched-chain α-ketoacid dehydrogenase complex has been cloned and sequenced. The amino acid sequence is highly conserved between rat, human and bovine forms of the protein. The β subunits of the branched-chain α-ketoacid dehydrogenase complex from rat and Pseudomonas putida, and the pyruvate dehydrogenase complex from human and Bacillus subtilis show sequence similarities which are shared with two other thiamine pyrophosphate-dependent enzymes, yeast (Hansenula polymorpha) formaldehyde transketolase and human transketolase. These similarities suggest that the β subunits may be involved in thiamine pyrophosphate binding.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 207-210 |
| Number of pages | 4 |
| Journal | BBA - Gene Structure and Expression |
| Volume | 1132 |
| Issue number | 2 |
| DOIs | |
| State | Published - Sep 24 1992 |
Keywords
- Branched chain α-ketoacid dehydrogenase
- cDNA
- Polymerase chain reaction
- Thiamine pyrophosphate
- α-Ketoacid dehydrogenase
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Genetics
- Structural Biology
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Zhao, Y., Kuntz, M. J., Harris, R. A., & Crabb, D. W. (1992). Molecular cloning of the E1β subunit of the rat branched chain α-ketoacid dehydrogenase. BBA - Gene Structure and Expression, 1132(2), 207-210. https://doi.org/10.1016/0167-4781(92)90014-Q