Molecular cloning of the E1β subunit of the rat branched chain α-ketoacid dehydrogenase

Y. Zhao, M. J. Kuntz, Robert Harris, David Crabb

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The rat liver E1β subunit of the branched-chain α-ketoacid dehydrogenase complex has been cloned and sequenced. The amino acid sequence is highly conserved between rat, human and bovine forms of the protein. The β subunits of the branched-chain α-ketoacid dehydrogenase complex from rat and Pseudomonas putida, and the pyruvate dehydrogenase complex from human and Bacillus subtilis show sequence similarities which are shared with two other thiamine pyrophosphate-dependent enzymes, yeast (Hansenula polymorpha) formaldehyde transketolase and human transketolase. These similarities suggest that the β subunits may be involved in thiamine pyrophosphate binding.

Original languageEnglish
Pages (from-to)207-210
Number of pages4
JournalBiochimica et Biophysica Acta - Gene Structure and Expression
Volume1132
Issue number2
DOIs
StatePublished - Sep 24 1992

Fingerprint

3-Methyl-2-Oxobutanoate Dehydrogenase (Lipoamide)
Cloning
Molecular Cloning
Thiamine Pyrophosphate
Rats
formaldehyde transketolase
Transketolase
Pyruvate Dehydrogenase Complex
Pseudomonas putida
Pichia
Bacilli
Bacillus subtilis
Liver
Yeast
Amino Acid Sequence
Yeasts
Amino Acids
Enzymes
Proteins

Keywords

  • Branched chain α-ketoacid dehydrogenase
  • cDNA
  • Polymerase chain reaction
  • Thiamine pyrophosphate
  • α-Ketoacid dehydrogenase

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Genetics
  • Structural Biology

Cite this

Molecular cloning of the E1β subunit of the rat branched chain α-ketoacid dehydrogenase. / Zhao, Y.; Kuntz, M. J.; Harris, Robert; Crabb, David.

In: Biochimica et Biophysica Acta - Gene Structure and Expression, Vol. 1132, No. 2, 24.09.1992, p. 207-210.

Research output: Contribution to journalArticle

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