Molecular cloning of the E1β subunit of the rat branched chain α-ketoacid dehydrogenase

Y. Zhao, M. J. Kuntz, R. A. Harris, D. W. Crabb

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

The rat liver E1β subunit of the branched-chain α-ketoacid dehydrogenase complex has been cloned and sequenced. The amino acid sequence is highly conserved between rat, human and bovine forms of the protein. The β subunits of the branched-chain α-ketoacid dehydrogenase complex from rat and Pseudomonas putida, and the pyruvate dehydrogenase complex from human and Bacillus subtilis show sequence similarities which are shared with two other thiamine pyrophosphate-dependent enzymes, yeast (Hansenula polymorpha) formaldehyde transketolase and human transketolase. These similarities suggest that the β subunits may be involved in thiamine pyrophosphate binding.

Original languageEnglish (US)
Pages (from-to)207-210
Number of pages4
JournalBBA - Gene Structure and Expression
Volume1132
Issue number2
DOIs
StatePublished - Sep 24 1992

Keywords

  • Branched chain α-ketoacid dehydrogenase
  • cDNA
  • Polymerase chain reaction
  • Thiamine pyrophosphate
  • α-Ketoacid dehydrogenase

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Genetics
  • Structural Biology

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