Molecular cloning of the E1β subunit of the rat branched chain α-ketoacid dehydrogenase

Y. Zhao; M. J. Kuntz; R. A. Harris; D. W. Crabb

doi:10.1016/0167-4781(92)90014-Q

Molecular cloning of the E1β subunit of the rat branched chain α-ketoacid dehydrogenase

Y. Zhao, M. J. Kuntz, R. A. Harris, D. W. Crabb

Research output: Contribution to journal › Article

9 Scopus citations

Abstract

The rat liver E1β subunit of the branched-chain α-ketoacid dehydrogenase complex has been cloned and sequenced. The amino acid sequence is highly conserved between rat, human and bovine forms of the protein. The β subunits of the branched-chain α-ketoacid dehydrogenase complex from rat and Pseudomonas putida, and the pyruvate dehydrogenase complex from human and Bacillus subtilis show sequence similarities which are shared with two other thiamine pyrophosphate-dependent enzymes, yeast (Hansenula polymorpha) formaldehyde transketolase and human transketolase. These similarities suggest that the β subunits may be involved in thiamine pyrophosphate binding.

Original language	English (US)
Pages (from-to)	207-210
Number of pages	4
Journal	BBA - Gene Structure and Expression
Volume	1132
Issue number	2
DOIs	https://doi.org/10.1016/0167-4781(92)90014-Q
State	Published - Sep 24 1992

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Keywords

Branched chain α-ketoacid dehydrogenase
cDNA
Polymerase chain reaction
Thiamine pyrophosphate
α-Ketoacid dehydrogenase

ASJC Scopus subject areas

Biochemistry
Biophysics
Genetics
Structural Biology

Cite this

Zhao, Y., Kuntz, M. J., Harris, R. A., & Crabb, D. W. (1992). Molecular cloning of the E1β subunit of the rat branched chain α-ketoacid dehydrogenase. BBA - Gene Structure and Expression, 1132(2), 207-210. https://doi.org/10.1016/0167-4781(92)90014-Q

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abstract = "The rat liver E1β subunit of the branched-chain α-ketoacid dehydrogenase complex has been cloned and sequenced. The amino acid sequence is highly conserved between rat, human and bovine forms of the protein. The β subunits of the branched-chain α-ketoacid dehydrogenase complex from rat and Pseudomonas putida, and the pyruvate dehydrogenase complex from human and Bacillus subtilis show sequence similarities which are shared with two other thiamine pyrophosphate-dependent enzymes, yeast (Hansenula polymorpha) formaldehyde transketolase and human transketolase. These similarities suggest that the β subunits may be involved in thiamine pyrophosphate binding.",

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10.1016/0167-4781(92)90014-Q