Molecular conservation of the P6 outer membrane protein among strains of Haemophilus influenzae

Analysis of antigenic determinants, gene sequences, and restriction fragment length polymorphisms

M. B. Nelson, R. S. Munson, M. A. Apicella, D. J. Sikkema, Jean Molleston, T. F. Murphy

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

Infections caused by Haemophilus influenzae are a major worldwide health problem. In particular, nontypeable strains of H. influenzae are a common cause of otitis media in infants and children. A vaccine to prevent these infections would result in the prevention of substantial morbidity and cost savings. A problem in identifying an appropriate vaccine antigen has been the enormous antigenic heterogeneity among nontypeable strains of H. influenzae. The present study was undertaken to characterize the conservation of the P6 outer membrane protein (~16,000 daltons) among strains of H. influenzae. A total of 20 type b strains and 20 nontypeable strains of diverse geographic and clinical origins was studied. Three approaches were taken. (i) Antigenic determinants recognized by monoclonal and polyclonal antibodies were present on P6 in all 40 strains tested. The molecular weight of P6 was identical in all strains. (ii) Comparison of the DNA sequences of the P6 genes from three epidemiologically and serologically unrelated strains demonstrated 100% homology at the amino acid level and 97 to 99% homology at the nucleotide level. (iii) Restriction fragment length polymorphism analysis demonstrated that the P6 gene and flanking sequences were highly conserved among all strains. These three independent series of experiments indicated that the P6 protein is highly conserved among strains of H. influenzae. P6 should receive serious consideration for inclusion in a vaccine to prevent infections caused by nontypeable H. influenzae.

Original languageEnglish (US)
Pages (from-to)2658-2663
Number of pages6
JournalInfection and Immunity
Volume59
Issue number8
StatePublished - 1991
Externally publishedYes

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Haemophilus influenzae
Restriction Fragment Length Polymorphisms
Epitopes
Membrane Proteins
Genes
Vaccines
Infection
Cost Savings
Otitis Media
Nucleotides
Molecular Weight
Monoclonal Antibodies
Morbidity
Antigens
Amino Acids
Proteins

ASJC Scopus subject areas

  • Immunology

Cite this

Molecular conservation of the P6 outer membrane protein among strains of Haemophilus influenzae : Analysis of antigenic determinants, gene sequences, and restriction fragment length polymorphisms. / Nelson, M. B.; Munson, R. S.; Apicella, M. A.; Sikkema, D. J.; Molleston, Jean; Murphy, T. F.

In: Infection and Immunity, Vol. 59, No. 8, 1991, p. 2658-2663.

Research output: Contribution to journalArticle

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