Molecular evidence for the independent origin of extra-ovarian papillary serous tumors of low malignant potential

Jian Gu, Lawrence M. Roth, Cheryl Younger, Helen Michael, Fadi W. Abdul-Karim, Shaobo Zhang, Thomas Ulbright, John Eble, Liang Cheng

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Abstract

Background: Molecular data suggest that peritoneal tumors in women with advanced-stage ovarian papillary serous adenocarcinoma are monoclonal in origin. Whether the same is true for ovarian tumors of low malignant potentials is not known. We compared peritoneal and ovarian tumors from women with advanced-stage ovarian papillary serous tumors of low malignant potential determine whether the peritoneal tumors arose from the same clone as the ovarian tumors. Methods: We studied the clonality of 73 peritoneal and ovarian tumors from 18 women with advanced-stage ovarian papillary serous tumors of low malignant potential. Formalin-fixed, paraffin- embedded tumors and representative normal tissues were sectioned and stained with hematoxylin-eosin, representative sections from separate tumors were manually microdissected, genomic DNA was extracted from the microdissected tumors, and the polymerase chain reaction was used to amplify a CAG polymorphic site in the human androgen receptor locus on the X chromosome to determine the inactivation pattern of X chromosome and the clonality of the tumors. Results: The pattern of X-chromosome inactivation could be determined from the tumors of 13 of 18 patients. Of the 13 patients, seven (54%) had nonrandom inactivation of the X chromosome, and six of the seven had different inactivation patterns in the peritoneal and ovarian tumors. Three of these patients also had different patterns of nonrandom X-chromosome inactivation in tumors from each ovary. The remaining six patients had random patterns of X- chromosome inactivation in the peritoneal and ovarian tumors. Conclusions: Our data suggest that peritoneal and ovarian tumors of low malignant potential arise independently.

Original languageEnglish
Pages (from-to)1147-1152
Number of pages6
JournalJournal of the National Cancer Institute
Volume93
Issue number15
StatePublished - Aug 1 2001

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Neoplasms
X Chromosome Inactivation
Papillary Adenocarcinoma
X Chromosome
Hematoxylin
Eosine Yellowish-(YS)
Paraffin
Formaldehyde
Ovary
Clone Cells
Polymerase Chain Reaction
DNA

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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Molecular evidence for the independent origin of extra-ovarian papillary serous tumors of low malignant potential. / Gu, Jian; Roth, Lawrence M.; Younger, Cheryl; Michael, Helen; Abdul-Karim, Fadi W.; Zhang, Shaobo; Ulbright, Thomas; Eble, John; Cheng, Liang.

In: Journal of the National Cancer Institute, Vol. 93, No. 15, 01.08.2001, p. 1147-1152.

Research output: Contribution to journalArticle

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abstract = "Background: Molecular data suggest that peritoneal tumors in women with advanced-stage ovarian papillary serous adenocarcinoma are monoclonal in origin. Whether the same is true for ovarian tumors of low malignant potentials is not known. We compared peritoneal and ovarian tumors from women with advanced-stage ovarian papillary serous tumors of low malignant potential determine whether the peritoneal tumors arose from the same clone as the ovarian tumors. Methods: We studied the clonality of 73 peritoneal and ovarian tumors from 18 women with advanced-stage ovarian papillary serous tumors of low malignant potential. Formalin-fixed, paraffin- embedded tumors and representative normal tissues were sectioned and stained with hematoxylin-eosin, representative sections from separate tumors were manually microdissected, genomic DNA was extracted from the microdissected tumors, and the polymerase chain reaction was used to amplify a CAG polymorphic site in the human androgen receptor locus on the X chromosome to determine the inactivation pattern of X chromosome and the clonality of the tumors. Results: The pattern of X-chromosome inactivation could be determined from the tumors of 13 of 18 patients. Of the 13 patients, seven (54{\%}) had nonrandom inactivation of the X chromosome, and six of the seven had different inactivation patterns in the peritoneal and ovarian tumors. Three of these patients also had different patterns of nonrandom X-chromosome inactivation in tumors from each ovary. The remaining six patients had random patterns of X- chromosome inactivation in the peritoneal and ovarian tumors. Conclusions: Our data suggest that peritoneal and ovarian tumors of low malignant potential arise independently.",
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AU - Gu, Jian

AU - Roth, Lawrence M.

AU - Younger, Cheryl

AU - Michael, Helen

AU - Abdul-Karim, Fadi W.

AU - Zhang, Shaobo

AU - Ulbright, Thomas

AU - Eble, John

AU - Cheng, Liang

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N2 - Background: Molecular data suggest that peritoneal tumors in women with advanced-stage ovarian papillary serous adenocarcinoma are monoclonal in origin. Whether the same is true for ovarian tumors of low malignant potentials is not known. We compared peritoneal and ovarian tumors from women with advanced-stage ovarian papillary serous tumors of low malignant potential determine whether the peritoneal tumors arose from the same clone as the ovarian tumors. Methods: We studied the clonality of 73 peritoneal and ovarian tumors from 18 women with advanced-stage ovarian papillary serous tumors of low malignant potential. Formalin-fixed, paraffin- embedded tumors and representative normal tissues were sectioned and stained with hematoxylin-eosin, representative sections from separate tumors were manually microdissected, genomic DNA was extracted from the microdissected tumors, and the polymerase chain reaction was used to amplify a CAG polymorphic site in the human androgen receptor locus on the X chromosome to determine the inactivation pattern of X chromosome and the clonality of the tumors. Results: The pattern of X-chromosome inactivation could be determined from the tumors of 13 of 18 patients. Of the 13 patients, seven (54%) had nonrandom inactivation of the X chromosome, and six of the seven had different inactivation patterns in the peritoneal and ovarian tumors. Three of these patients also had different patterns of nonrandom X-chromosome inactivation in tumors from each ovary. The remaining six patients had random patterns of X- chromosome inactivation in the peritoneal and ovarian tumors. Conclusions: Our data suggest that peritoneal and ovarian tumors of low malignant potential arise independently.

AB - Background: Molecular data suggest that peritoneal tumors in women with advanced-stage ovarian papillary serous adenocarcinoma are monoclonal in origin. Whether the same is true for ovarian tumors of low malignant potentials is not known. We compared peritoneal and ovarian tumors from women with advanced-stage ovarian papillary serous tumors of low malignant potential determine whether the peritoneal tumors arose from the same clone as the ovarian tumors. Methods: We studied the clonality of 73 peritoneal and ovarian tumors from 18 women with advanced-stage ovarian papillary serous tumors of low malignant potential. Formalin-fixed, paraffin- embedded tumors and representative normal tissues were sectioned and stained with hematoxylin-eosin, representative sections from separate tumors were manually microdissected, genomic DNA was extracted from the microdissected tumors, and the polymerase chain reaction was used to amplify a CAG polymorphic site in the human androgen receptor locus on the X chromosome to determine the inactivation pattern of X chromosome and the clonality of the tumors. Results: The pattern of X-chromosome inactivation could be determined from the tumors of 13 of 18 patients. Of the 13 patients, seven (54%) had nonrandom inactivation of the X chromosome, and six of the seven had different inactivation patterns in the peritoneal and ovarian tumors. Three of these patients also had different patterns of nonrandom X-chromosome inactivation in tumors from each ovary. The remaining six patients had random patterns of X- chromosome inactivation in the peritoneal and ovarian tumors. Conclusions: Our data suggest that peritoneal and ovarian tumors of low malignant potential arise independently.

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