Molecular evidence supporting the neoplastic nature of odontogenic keratocyst: A laser capture microdissection study of 15 cases

John D. Henley, D. J. Summerlin, C. Tomich, Shaobo Zhang, Liang Cheng

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

Aims: The bland histology of odontogenic keratocyst (OKC) belies its capacity for aggressive behaviour. Genetic alterations of OKC have not been well studied. We examined the frequency and pattern of allelic imbalance on five different chromosome regions from 15 patients with OKC. Methods and results: Laser-assisted microdissection was performed on formalin-fixed paraffin-embedded tissue. Polymerase chain reaction analysis of extracted DNA targeted five polymorphic DNA markers (D3S1285, D9S161, D11S1316, D13S290, and TP53) representing chromosome regions 3p14, 9p21, 11q23, 13q12.1 and 17p13, respectively. All 15 cases of OKC were informative at a minimum of three of five loci, with 11 informative on all five loci. Twelve of 15 cases (80%) demonstrated loss of heterozygosity (LOH). Seven cases (47%) showed LOH at more than two DNA loci. The frequency of LOH was 5/11 (45%) at D3S1285, 3/15 (20%) at D9S161, 4/14 (29%) at D11S1316, 8/14 (57%) at D13S290 and 3/15 (20%) at TP53. Conclusions: The majority of OKCs harbour chromosomal abnormalities. This finding supports the supposition that OKCs are neoplastic. Furthermore, OKCs harbour allelic loss at some of the same loci identified in squamous cell carcinoma. This may aid in explaining the rare occurrence of squamous cell carcinoma arising in OKC.

Original languageEnglish
Pages (from-to)582-586
Number of pages5
JournalHistopathology
Volume47
Issue number6
DOIs
StatePublished - Dec 2005

Fingerprint

Odontogenic Cysts
Laser Capture Microdissection
Loss of Heterozygosity
Squamous Cell Carcinoma
Allelic Imbalance
Chromosomes, Human, Pair 15
Microdissection
DNA
Genetic Markers
Chromosome Aberrations
Paraffin
Formaldehyde
Histology
Lasers
Chromosomes
Polymerase Chain Reaction

Keywords

  • Clonality
  • Laser capture
  • Loss of heterozygosity
  • Microdissection
  • Odontogenic keratocyst

ASJC Scopus subject areas

  • Anatomy
  • Pathology and Forensic Medicine
  • Cell Biology

Cite this

Molecular evidence supporting the neoplastic nature of odontogenic keratocyst : A laser capture microdissection study of 15 cases. / Henley, John D.; Summerlin, D. J.; Tomich, C.; Zhang, Shaobo; Cheng, Liang.

In: Histopathology, Vol. 47, No. 6, 12.2005, p. 582-586.

Research output: Contribution to journalArticle

@article{ca4a972f23294cb9bf89e1842dc80f6c,
title = "Molecular evidence supporting the neoplastic nature of odontogenic keratocyst: A laser capture microdissection study of 15 cases",
abstract = "Aims: The bland histology of odontogenic keratocyst (OKC) belies its capacity for aggressive behaviour. Genetic alterations of OKC have not been well studied. We examined the frequency and pattern of allelic imbalance on five different chromosome regions from 15 patients with OKC. Methods and results: Laser-assisted microdissection was performed on formalin-fixed paraffin-embedded tissue. Polymerase chain reaction analysis of extracted DNA targeted five polymorphic DNA markers (D3S1285, D9S161, D11S1316, D13S290, and TP53) representing chromosome regions 3p14, 9p21, 11q23, 13q12.1 and 17p13, respectively. All 15 cases of OKC were informative at a minimum of three of five loci, with 11 informative on all five loci. Twelve of 15 cases (80{\%}) demonstrated loss of heterozygosity (LOH). Seven cases (47{\%}) showed LOH at more than two DNA loci. The frequency of LOH was 5/11 (45{\%}) at D3S1285, 3/15 (20{\%}) at D9S161, 4/14 (29{\%}) at D11S1316, 8/14 (57{\%}) at D13S290 and 3/15 (20{\%}) at TP53. Conclusions: The majority of OKCs harbour chromosomal abnormalities. This finding supports the supposition that OKCs are neoplastic. Furthermore, OKCs harbour allelic loss at some of the same loci identified in squamous cell carcinoma. This may aid in explaining the rare occurrence of squamous cell carcinoma arising in OKC.",
keywords = "Clonality, Laser capture, Loss of heterozygosity, Microdissection, Odontogenic keratocyst",
author = "Henley, {John D.} and Summerlin, {D. J.} and C. Tomich and Shaobo Zhang and Liang Cheng",
year = "2005",
month = "12",
doi = "10.1111/j.1365-2559.2005.02267.x",
language = "English",
volume = "47",
pages = "582--586",
journal = "Histopathology",
issn = "0309-0167",
publisher = "Wiley-Blackwell",
number = "6",

}

TY - JOUR

T1 - Molecular evidence supporting the neoplastic nature of odontogenic keratocyst

T2 - A laser capture microdissection study of 15 cases

AU - Henley, John D.

AU - Summerlin, D. J.

AU - Tomich, C.

AU - Zhang, Shaobo

AU - Cheng, Liang

PY - 2005/12

Y1 - 2005/12

N2 - Aims: The bland histology of odontogenic keratocyst (OKC) belies its capacity for aggressive behaviour. Genetic alterations of OKC have not been well studied. We examined the frequency and pattern of allelic imbalance on five different chromosome regions from 15 patients with OKC. Methods and results: Laser-assisted microdissection was performed on formalin-fixed paraffin-embedded tissue. Polymerase chain reaction analysis of extracted DNA targeted five polymorphic DNA markers (D3S1285, D9S161, D11S1316, D13S290, and TP53) representing chromosome regions 3p14, 9p21, 11q23, 13q12.1 and 17p13, respectively. All 15 cases of OKC were informative at a minimum of three of five loci, with 11 informative on all five loci. Twelve of 15 cases (80%) demonstrated loss of heterozygosity (LOH). Seven cases (47%) showed LOH at more than two DNA loci. The frequency of LOH was 5/11 (45%) at D3S1285, 3/15 (20%) at D9S161, 4/14 (29%) at D11S1316, 8/14 (57%) at D13S290 and 3/15 (20%) at TP53. Conclusions: The majority of OKCs harbour chromosomal abnormalities. This finding supports the supposition that OKCs are neoplastic. Furthermore, OKCs harbour allelic loss at some of the same loci identified in squamous cell carcinoma. This may aid in explaining the rare occurrence of squamous cell carcinoma arising in OKC.

AB - Aims: The bland histology of odontogenic keratocyst (OKC) belies its capacity for aggressive behaviour. Genetic alterations of OKC have not been well studied. We examined the frequency and pattern of allelic imbalance on five different chromosome regions from 15 patients with OKC. Methods and results: Laser-assisted microdissection was performed on formalin-fixed paraffin-embedded tissue. Polymerase chain reaction analysis of extracted DNA targeted five polymorphic DNA markers (D3S1285, D9S161, D11S1316, D13S290, and TP53) representing chromosome regions 3p14, 9p21, 11q23, 13q12.1 and 17p13, respectively. All 15 cases of OKC were informative at a minimum of three of five loci, with 11 informative on all five loci. Twelve of 15 cases (80%) demonstrated loss of heterozygosity (LOH). Seven cases (47%) showed LOH at more than two DNA loci. The frequency of LOH was 5/11 (45%) at D3S1285, 3/15 (20%) at D9S161, 4/14 (29%) at D11S1316, 8/14 (57%) at D13S290 and 3/15 (20%) at TP53. Conclusions: The majority of OKCs harbour chromosomal abnormalities. This finding supports the supposition that OKCs are neoplastic. Furthermore, OKCs harbour allelic loss at some of the same loci identified in squamous cell carcinoma. This may aid in explaining the rare occurrence of squamous cell carcinoma arising in OKC.

KW - Clonality

KW - Laser capture

KW - Loss of heterozygosity

KW - Microdissection

KW - Odontogenic keratocyst

UR - http://www.scopus.com/inward/record.url?scp=28844482638&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=28844482638&partnerID=8YFLogxK

U2 - 10.1111/j.1365-2559.2005.02267.x

DO - 10.1111/j.1365-2559.2005.02267.x

M3 - Article

C2 - 16324195

AN - SCOPUS:28844482638

VL - 47

SP - 582

EP - 586

JO - Histopathology

JF - Histopathology

SN - 0309-0167

IS - 6

ER -