Molecular evidence that in situ-transduced fetal liver hematopoietic stem/progenitor cells give rise to medullary hematopoiesis in adult rats

D. Clapp, Brian Freie, Wei Hua Lee, You Yan Zhang

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

We exploited the ability to transduce fetal liver hematopoietic stem/progenitor cells in situ with recombinant retrovirus, together with the ability to analyze proviral integration patterns into chromosomal DNA, to detect the cellular and organ fate of hematopoietic stem and progenitor- derived progeny in tissues and in the circulation of neonatal and adult rats. Two hundred seventeen fetuses were injected with retrovirus supernatant on day 16 of gestation, before the development of the bone marrow cavity. The progeny of 41 stem and progenitor cella from 97 liveborn rats were clonally identified. Pluripotent and lineage-restricted stem/progenitor clones derived from the fetal liver consistently gave rise to progeny in the marrow of newborn and adult rats. Patterns of differentiation of transduced stem and progenitor cells fell into distinct subsets. Blood cells derived from in situ transduced cells that originated in the fetal liver circulated throughout the life span of the adult animals. These data provide molecular evidence of the origin of medullary cavity hematopoiesis by cells derived from the fetal liver that were transduced in vivo, homed to the developing medullary cavity and proliferated in a normal medullary hematopoietic microenvironment.

Original languageEnglish
Pages (from-to)2113-2122
Number of pages10
JournalBlood
Volume86
Issue number6
StatePublished - Sep 15 1995

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Hematopoiesis
Hematopoietic Stem Cells
Stem cells
Liver
Rats
Retroviridae
Stem Cells
Bone Marrow
Blood Cells
Bone
Animals
Fetus
Blood
Clone Cells
Cells
Tissue
Pregnancy
DNA

ASJC Scopus subject areas

  • Hematology

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Molecular evidence that in situ-transduced fetal liver hematopoietic stem/progenitor cells give rise to medullary hematopoiesis in adult rats. / Clapp, D.; Freie, Brian; Lee, Wei Hua; Zhang, You Yan.

In: Blood, Vol. 86, No. 6, 15.09.1995, p. 2113-2122.

Research output: Contribution to journalArticle

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